pBR322Amy
pBR322Amy
规格:
货期:
编号:TS146391
品牌:Testobio
| 产品名称： | pBR322Amy |
|---|---|
| 商品货号： | TS146391 |
| Designations： | pBR322Amy |
| Species： | Geobacillus stearothermophilus (Donk) Nazina et al. |
| Depositors： | CPC International, Inc. |
| Applications： | produces protein amylase, alpha 1A; salivary Amylase, salivary, alpha-1A |
| Vector： | Construct size (kb): 9.899999618530273 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: HindIII Vector end: HindIII Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: HindIII Vector end: HindIII Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Bacillus stearothermophilus Gene name: alpha-amylase Contains complete coding sequence?: U Strain: N21 Type of DNA: genomic Insert end: HindIII Insert end: HindIII Insert size (kb): 5.3 Cross references: DESCRIPTION OF INSERT COMPONENT: Genome: Bacillus stearothermophilus Gene name: alpha-amylase Contains complete coding sequence?: U Strain: N21 Type of DNA: genomic Insert end: HindIII Insert end: HindIII Insert size (kb): 5.3 Cross references: Insert lengths(kb): 5.300000190734863 Gene product: amylase, alpha 1A; salivary(Amylase, salivary, alpha-1A) Target Gene: amylase, alpha 1A; salivary |
| Insert Size (kb)： | 5.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--7.4, 1.2 (doublet); HindIII--5.3, 4.6; PstI--7.4, 2.5. The plasmid encodes a thermostable alpha-amylase which is produced intracellularly and not exported. |
| References： | Mielenz JR, Mickel S. Process for cloning the gene coding for a thermostable alpha-amylase into Escherichia coli and Bacillus subtilis. US Patent 4,493,893 dated Jan 15 1985 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pBR322Amy |
|---|---|
| 商品货号： | TS146391 |
| Designations： | pBR322Amy |
| Species： | Geobacillus stearothermophilus (Donk) Nazina et al. |
| Depositors： | CPC International, Inc. |
| Applications： | produces protein amylase, alpha 1A; salivary Amylase, salivary, alpha-1A |
| Vector： | Construct size (kb): 9.899999618530273 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: HindIII Vector end: HindIII Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: HindIII Vector end: HindIII Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Bacillus stearothermophilus Gene name: alpha-amylase Contains complete coding sequence?: U Strain: N21 Type of DNA: genomic Insert end: HindIII Insert end: HindIII Insert size (kb): 5.3 Cross references: DESCRIPTION OF INSERT COMPONENT: Genome: Bacillus stearothermophilus Gene name: alpha-amylase Contains complete coding sequence?: U Strain: N21 Type of DNA: genomic Insert end: HindIII Insert end: HindIII Insert size (kb): 5.3 Cross references: Insert lengths(kb): 5.300000190734863 Gene product: amylase, alpha 1A; salivary(Amylase, salivary, alpha-1A) Target Gene: amylase, alpha 1A; salivary |
| Insert Size (kb)： | 5.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--7.4, 1.2 (doublet); HindIII--5.3, 4.6; PstI--7.4, 2.5. The plasmid encodes a thermostable alpha-amylase which is produced intracellularly and not exported. |
| References： | Mielenz JR, Mickel S. Process for cloning the gene coding for a thermostable alpha-amylase into Escherichia coli and Bacillus subtilis. US Patent 4,493,893 dated Jan 15 1985 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
