| 产品名称： | pBKE |
|---|---|
| 商品货号： | TS146409 |
| Designations： | pBKE |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 4.03000020980835 |
| Insert： | DNA: Synthetic Insert lengths(kb): 0.0390000008046627 Gene product: immunoglobulin kappa enhancer IGK* |
| Insert Size (kb)： | 0.039 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.1; EcoRI--4.1; PvuII--4.1; HindIII--uncut; EcoRI/BamHI--4.1. In 70Z/3 cells, kappa enhancer binding activity was absent in unstimulated cells, but was induced in cells incubated with lipopolysaccharide and interleukin-1, forskolin, or BrcAMP. The sequence of the insert, from the EcoRI to the BamHI sites is: GAATTCGGCA TCTCAACAGA GGGGACTTTC CGAGAGGCGG ATCC. The string of 4 Gs begins the 11 bp NF-kappaB-binding site. |
| References： | Shirakawa F, Mizel SB. In vitro activation and nuclear translocation of NF-kappaB catalyzed by cyclin AMP-dependent protein kinase and protein kinase C. Mol. Cell. Biol. 9: 2424-2430, 1989. PubMed: 2548081 Shirakawa F, et al. Interleukin 1 and cyclic AMP induce kappa immunoglobulin light-chain expression via activation of an NF-kappaB-like DNA-binding protein. Mol. Cell. Biol. 9: 959-964, 1989. PubMed: 2542770 |
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| 产品名称： | pBKE |
|---|---|
| 商品货号： | TS146409 |
| Designations： | pBKE |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 4.03000020980835 |
| Insert： | DNA: Synthetic Insert lengths(kb): 0.0390000008046627 Gene product: immunoglobulin kappa enhancer IGK* |
| Insert Size (kb)： | 0.039 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.1; EcoRI--4.1; PvuII--4.1; HindIII--uncut; EcoRI/BamHI--4.1. In 70Z/3 cells, kappa enhancer binding activity was absent in unstimulated cells, but was induced in cells incubated with lipopolysaccharide and interleukin-1, forskolin, or BrcAMP. The sequence of the insert, from the EcoRI to the BamHI sites is: GAATTCGGCA TCTCAACAGA GGGGACTTTC CGAGAGGCGG ATCC. The string of 4 Gs begins the 11 bp NF-kappaB-binding site. |
| References： | Shirakawa F, Mizel SB. In vitro activation and nuclear translocation of NF-kappaB catalyzed by cyclin AMP-dependent protein kinase and protein kinase C. Mol. Cell. Biol. 9: 2424-2430, 1989. PubMed: 2548081 Shirakawa F, et al. Interleukin 1 and cyclic AMP induce kappa immunoglobulin light-chain expression via activation of an NF-kappaB-like DNA-binding protein. Mol. Cell. Biol. 9: 959-964, 1989. PubMed: 2542770 |
