HFH 15-9-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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HFH 15-9
HFH 15-9
规格:
货期:
编号:TS146680
品牌:Testobio
产品名称: HFH 15-9
商品货号: TS146680
Designations: HFH 15-9
GenBank Number:

U16861

Species: Homo sapiens, human
Depositors: L Wood
Vector:
Construct size (kb): 45.29999923706055
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdagt11
Intact vector size: 43.700
Type of vector: phage
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Cloning capacity: 7.2 kb
Construction: lambdagt7-lac5(b522 nin5); lambdagt4(cI857 S100 nin5)
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ
promoter: lac
replicon: lambda
repressor gene: cI857
Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: KCNJ2
Other gene symbols: IRK1
Gene name: potassium inwardly-rectifying channel, subfamily J, member
2
Contains complete coding sequence?: Y
Tissue: fetal heart
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 1.671
Cross references: DNA Seq. Acc.: U16861
Nucleotides 1-1671 of the insert correspond to
nucleotides 1-1671 of U16861.
Insert lengths(kb): 1.671000003814697
Tissue: fetal heart
Gene product: potassium inwardly-rectifying channel, subfamily J, member 2 IRK1
Insert Size (kb): 1.671
Media: ATCC® Medium 1592: SM buffer
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: Frozen
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI-26.0, 20.0; HindIII--25.0, 21.0, 9.5, 5.6; XbaI--25.0, 22.0, 19.0.
The insert contains the following restriction sites (approximate kb from the 5 end): KpnI--0.53; HindIII--0.69; ClaI--0.84, 1.10; XbaI--1.57.
References:

Wood LS, et al. Cloning and functional expression of a human gene, hIRK1, encoding the heart inward rectifier K+-channel. Gene 163: 313-317, 1995. PubMed: 7590287

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HFH 15-9

  • 货号: TS146680
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: HFH 15-9
商品货号: TS146680
Designations: HFH 15-9
GenBank Number:

U16861

Species: Homo sapiens, human
Depositors: L Wood
Vector:
Construct size (kb): 45.29999923706055
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdagt11
Intact vector size: 43.700
Type of vector: phage
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Cloning capacity: 7.2 kb
Construction: lambdagt7-lac5(b522 nin5); lambdagt4(cI857 S100 nin5)
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ
promoter: lac
replicon: lambda
repressor gene: cI857
Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: KCNJ2
Other gene symbols: IRK1
Gene name: potassium inwardly-rectifying channel, subfamily J, member
2
Contains complete coding sequence?: Y
Tissue: fetal heart
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 1.671
Cross references: DNA Seq. Acc.: U16861
Nucleotides 1-1671 of the insert correspond to
nucleotides 1-1671 of U16861.
Insert lengths(kb): 1.671000003814697
Tissue: fetal heart
Gene product: potassium inwardly-rectifying channel, subfamily J, member 2 IRK1
Insert Size (kb): 1.671
Media: ATCC® Medium 1592: SM buffer
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: Frozen
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI-26.0, 20.0; HindIII--25.0, 21.0, 9.5, 5.6; XbaI--25.0, 22.0, 19.0.
The insert contains the following restriction sites (approximate kb from the 5 end): KpnI--0.53; HindIII--0.69; ClaI--0.84, 1.10; XbaI--1.57.
References:

Wood LS, et al. Cloning and functional expression of a human gene, hIRK1, encoding the heart inward rectifier K+-channel. Gene 163: 313-317, 1995. PubMed: 7590287

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