| 产品名称： | pMLC3/7 |
|---|---|
| 商品货号： | TS146812 |
| Designations： | pMLC3/7 |
| GenBank Number： | K02782 |
| Species： | Mus musculus, mouse |
| Depositors： | GH Fey |
| Vector： | Construct size (kb): 8.399999618530273 |
| Insert： | DNA: cDNA Insert lengths(kb): 4.0 Tissue: liver, adult male Gene product: complement component 3 C3 |
| Insert Size (kb)： | 4.0 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--7.6, 0.8; HindIII--7.7, 0.9; XbaI--8.4; PstI--5.9, 0.8, 0.7; BamHI--6.4, 2.0. The insert contains the following restriction sites (nucleotide positions according to GenBank K02782): EcoRI--342; HindIII--428; BamHI--1093; BglII--1897, 2027; StuI--1982, 2223, 4041; PstI--2011, 2676, 3511, 3607; PvuI--4017. One of the PstI sites on the insert was destroyed during cloning. The insert begins 5 of the EcoRI site at position 342 of the published sequence and extends 3 between the StuI site at 4041 and the SalI site at 4677. It encodes most of the beta chain (129-2054) and part of the alpha (2067-5045). |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Domdey H, et al. Characterization of the mRNA and cloned cDNA specifying the third component of mouse complement. Proc. Natl. Acad. Sci. USA 79: 7619-7623, 1982. PubMed: 6961437 Lundwall A, et al. Structure of murine complement component C3. I. Nucleotide sequence of cloned complementary and genomic DNA coding for the beta chain. J. Biol. Chem. 259: 13851-13856, 1984. PubMed: 6548745 Wetsel RA, et al. Structure of murine complement component C3. II. Nucleotide sequence of cloned complementary DNA coding for the alpha chain. J. Biol. Chem. 259: 13857-13862, 1984. PubMed: 6094532 Fey GH, et al. Nucleotide sequence of complementary DNA and derived amino acid sequence of murine complement protein C3. Philos. Trans. R. Soc. Lond. B Biol. Sci. 306: 333-344, 1984. PubMed: 6208565 Georg H Fey, personal communication |
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| 产品名称： | pMLC3/7 |
|---|---|
| 商品货号： | TS146812 |
| Designations： | pMLC3/7 |
| GenBank Number： | K02782 |
| Species： | Mus musculus, mouse |
| Depositors： | GH Fey |
| Vector： | Construct size (kb): 8.399999618530273 |
| Insert： | DNA: cDNA Insert lengths(kb): 4.0 Tissue: liver, adult male Gene product: complement component 3 C3 |
| Insert Size (kb)： | 4.0 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--7.6, 0.8; HindIII--7.7, 0.9; XbaI--8.4; PstI--5.9, 0.8, 0.7; BamHI--6.4, 2.0. The insert contains the following restriction sites (nucleotide positions according to GenBank K02782): EcoRI--342; HindIII--428; BamHI--1093; BglII--1897, 2027; StuI--1982, 2223, 4041; PstI--2011, 2676, 3511, 3607; PvuI--4017. One of the PstI sites on the insert was destroyed during cloning. The insert begins 5 of the EcoRI site at position 342 of the published sequence and extends 3 between the StuI site at 4041 and the SalI site at 4677. It encodes most of the beta chain (129-2054) and part of the alpha (2067-5045). |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Domdey H, et al. Characterization of the mRNA and cloned cDNA specifying the third component of mouse complement. Proc. Natl. Acad. Sci. USA 79: 7619-7623, 1982. PubMed: 6961437 Lundwall A, et al. Structure of murine complement component C3. I. Nucleotide sequence of cloned complementary and genomic DNA coding for the beta chain. J. Biol. Chem. 259: 13851-13856, 1984. PubMed: 6548745 Wetsel RA, et al. Structure of murine complement component C3. II. Nucleotide sequence of cloned complementary DNA coding for the alpha chain. J. Biol. Chem. 259: 13857-13862, 1984. PubMed: 6094532 Fey GH, et al. Nucleotide sequence of complementary DNA and derived amino acid sequence of murine complement protein C3. Philos. Trans. R. Soc. Lond. B Biol. Sci. 306: 333-344, 1984. PubMed: 6208565 Georg H Fey, personal communication |
