| 产品名称： | HIBBC63 |
|---|---|
| 商品货号： | TS146907 |
| Designations： | HIBBC63 |
| GenBank Number： | M25756 T08333 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 5.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: lafmid BA Intact vector size: 3.153 Type of vector: phagemid Vector end: HindIII Vector end: NotI Cloning sites: HindIII, BamHI, NotI, EcoRI Polylinker sites: HindIII, BamHI, NotI, EcoRI Other unique sites: ClaI, XmnI, ScaI, ApaLI Construction: pGEM Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, insert detection: lacZ, primer site: M13 universal, -> MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098 primer site: M13 reverse, promoter: lac, replicon: pMB1, marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: SCGR* Gene name: secretogranin II Contains complete coding sequence?: N Tissue: infant brain, 3 months old (from Bento Soares) Type of DNA: cDNA Insert 5 end: HindIII Insert 3 end: NotI Insert size (kb): 1.7 Cross references: DNA Seq. Acc.: M25756; EST: EST06224 Nucleotides 1-390 of the insert correspond to nucleotides 721-1110 of M25756 with 98% identity. DNA Seq. Acc.: T08333; EST: EST06224; Primer: M13 Reverse; THC id: THC85513 Insert lengths(kb): 1.700000047683716 Tissue: infant brain, 3 months old (from Bento Soares) Gene product: secretogranin II SCGR* |
| Insert Size (kb)： | 1.700 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII/NotI--3.3, 1.7, EcoRI--5.0. Based on the alignment with GenBank accession M25756 (2336 nt, CDS 63-1916), this clone appears to contain part of the coding sequence. The cDNAs (>95%) are cloned directionally with the 5 end adjacent to the M13 reverse primer and HindIII. |
| References： | Adams MD, et al. Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA library. Nat. Genet. 4: 373-380, 1993. PubMed: 8401585 Mark D Adams, personal communication |
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| 产品名称： | HIBBC63 |
|---|---|
| 商品货号： | TS146907 |
| Designations： | HIBBC63 |
| GenBank Number： | M25756 T08333 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 5.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: lafmid BA Intact vector size: 3.153 Type of vector: phagemid Vector end: HindIII Vector end: NotI Cloning sites: HindIII, BamHI, NotI, EcoRI Polylinker sites: HindIII, BamHI, NotI, EcoRI Other unique sites: ClaI, XmnI, ScaI, ApaLI Construction: pGEM Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, insert detection: lacZ, primer site: M13 universal, -> MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098 primer site: M13 reverse, promoter: lac, replicon: pMB1, marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: SCGR* Gene name: secretogranin II Contains complete coding sequence?: N Tissue: infant brain, 3 months old (from Bento Soares) Type of DNA: cDNA Insert 5 end: HindIII Insert 3 end: NotI Insert size (kb): 1.7 Cross references: DNA Seq. Acc.: M25756; EST: EST06224 Nucleotides 1-390 of the insert correspond to nucleotides 721-1110 of M25756 with 98% identity. DNA Seq. Acc.: T08333; EST: EST06224; Primer: M13 Reverse; THC id: THC85513 Insert lengths(kb): 1.700000047683716 Tissue: infant brain, 3 months old (from Bento Soares) Gene product: secretogranin II SCGR* |
| Insert Size (kb)： | 1.700 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII/NotI--3.3, 1.7, EcoRI--5.0. Based on the alignment with GenBank accession M25756 (2336 nt, CDS 63-1916), this clone appears to contain part of the coding sequence. The cDNAs (>95%) are cloned directionally with the 5 end adjacent to the M13 reverse primer and HindIII. |
| References： | Adams MD, et al. Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA library. Nat. Genet. 4: 373-380, 1993. PubMed: 8401585 Mark D Adams, personal communication |
