| 产品名称： | 45.1-p33-143 p33-143 |
|---|---|
| 商品货号： | TS147167 |
| Designations： | 45.1-p33-143 p33-143 |
| GenBank Number： | X00497 |
| Species： | Homo sapiens, human |
| Depositors： | EO Long |
| Vector： | Construct size (kb): 4.400000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.399999976158142 Tissue: hemizygous 45.1 cell line (DR1, DQw1, DPw2) Gene product: CD74 antigen (invariant polpypeptide of major histocompatibility class II antigen-associated)(invariant chain, HLA-DR associated) CD74 |
| Insert Size (kb)： | 1.400 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): SmaI/BamHI--3.2, 1.4; PstI--2.05, 1.2, 0.90, 0.37; PvuII--4.6; HindIII--2.9, 1.00, 0.80; BglI--2.9, 1.2, 0.58. The insert contains the following restriction sites (bp from the 5 SmaI site): PstI--290, 1159; PvuII--291; HindIII--310, 1266; BglI--399; NarI--129. A full-length cDNA clone. The ends of the SmaI/BamHI fragment are as follows: 5 SmaI site - GGGCGAGCT(G)15ATG......(A)n approximately 30 bp of SV40-BamHI - 3. |
| References： | Tonnelle C, et al. DObeta: a new beta chain gene in HLA-D with a distinct regulation of expression. EMBO J. 4: 2839-2847, 1985. PubMed: 2998758 Sekaly RP, et al. Cell surface expression of class II histocompatibility antigens occurs in the absence of the invariant chain. J. Exp. Med. 164: 1490-1504, 1986. PubMed: 2430042 Genuardi M, Saunders GF. Localization of the HLA class II-associated invariant chain to human chromosome band 5q32. Immunogenetics 28: 53-56, 1988. PubMed: 3132422 Strubin M, et al. Two forms of the Ia antigen-associated invariant chain result from alternative initiations at two in-phase AUGs. Cell 47: 619-625, 1986. PubMed: 3779839 Strubin M, et al. The complete sequence of the mRNA for the HLA-DR-associated invariant chain reveals a polypeptide with an unusual transmembrane polarity. EMBO J. 3: 869-872, 1984. PubMed: 6586420 Eric O Long, personal communication |
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| 产品名称： | 45.1-p33-143 p33-143 |
|---|---|
| 商品货号： | TS147167 |
| Designations： | 45.1-p33-143 p33-143 |
| GenBank Number： | X00497 |
| Species： | Homo sapiens, human |
| Depositors： | EO Long |
| Vector： | Construct size (kb): 4.400000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.399999976158142 Tissue: hemizygous 45.1 cell line (DR1, DQw1, DPw2) Gene product: CD74 antigen (invariant polpypeptide of major histocompatibility class II antigen-associated)(invariant chain, HLA-DR associated) CD74 |
| Insert Size (kb)： | 1.400 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): SmaI/BamHI--3.2, 1.4; PstI--2.05, 1.2, 0.90, 0.37; PvuII--4.6; HindIII--2.9, 1.00, 0.80; BglI--2.9, 1.2, 0.58. The insert contains the following restriction sites (bp from the 5 SmaI site): PstI--290, 1159; PvuII--291; HindIII--310, 1266; BglI--399; NarI--129. A full-length cDNA clone. The ends of the SmaI/BamHI fragment are as follows: 5 SmaI site - GGGCGAGCT(G)15ATG......(A)n approximately 30 bp of SV40-BamHI - 3. |
| References： | Tonnelle C, et al. DObeta: a new beta chain gene in HLA-D with a distinct regulation of expression. EMBO J. 4: 2839-2847, 1985. PubMed: 2998758 Sekaly RP, et al. Cell surface expression of class II histocompatibility antigens occurs in the absence of the invariant chain. J. Exp. Med. 164: 1490-1504, 1986. PubMed: 2430042 Genuardi M, Saunders GF. Localization of the HLA class II-associated invariant chain to human chromosome band 5q32. Immunogenetics 28: 53-56, 1988. PubMed: 3132422 Strubin M, et al. Two forms of the Ia antigen-associated invariant chain result from alternative initiations at two in-phase AUGs. Cell 47: 619-625, 1986. PubMed: 3779839 Strubin M, et al. The complete sequence of the mRNA for the HLA-DR-associated invariant chain reveals a polypeptide with an unusual transmembrane polarity. EMBO J. 3: 869-872, 1984. PubMed: 6586420 Eric O Long, personal communication |
