pTC4.5-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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pTC4.5
pTC4.5
规格:
货期:
编号:TS147420
品牌:Testobio
产品名称: pTC4.5
商品货号: TS147420
Designations: pTC4.5
GenBank Number:

L22484

Species: Trypanosoma cruzi Chagas
Depositors: LM Ruiz-Perez
Vector:
Construct size (kb): 7.190000057220459
Insert:
DNA: genomic
Insert lengths(kb): 4.5
Gene product: dihydrofolate reductase-thymidylate synthase DHFR-TS
Insert Size (kb): 4.5
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI/PstI--4.6, 2.8; HindIII--7.4; SalI--7.4.
The insert contains the following restriction sites (approximate from the 5 end): BstXI--3.95; NcoI--2.52; SalI--2.48; SphI--2.42, 2.76, 3.65; XhoI--2.79.
The insert contains the following components (listed 5->3): a PstI/SalI fragment from the MCS of lambda EMBL3, 2.4 kb upstream of the coding sequence for DHFR-TS, the 1.56 kb coding region, and 0.44 kb downstream of DHFR-TS.
All but 100 bp of the coding sequence is in a 1.9 kb SalI/EcoRI fragment.
Classification: KINGDOM: Protozoa
References:

Reche P, et al. Cloning and expression of the dihydrofolate reductase-thymidylate synthase gene from Trypanosoma cruzi. Mol. Biochem. Parasitol. 65: 247-258, 1994. PubMed: 7969266

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pTC4.5

  • 货号: TS147420
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: pTC4.5
商品货号: TS147420
Designations: pTC4.5
GenBank Number:

L22484

Species: Trypanosoma cruzi Chagas
Depositors: LM Ruiz-Perez
Vector:
Construct size (kb): 7.190000057220459
Insert:
DNA: genomic
Insert lengths(kb): 4.5
Gene product: dihydrofolate reductase-thymidylate synthase DHFR-TS
Insert Size (kb): 4.5
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI/PstI--4.6, 2.8; HindIII--7.4; SalI--7.4.
The insert contains the following restriction sites (approximate from the 5 end): BstXI--3.95; NcoI--2.52; SalI--2.48; SphI--2.42, 2.76, 3.65; XhoI--2.79.
The insert contains the following components (listed 5->3): a PstI/SalI fragment from the MCS of lambda EMBL3, 2.4 kb upstream of the coding sequence for DHFR-TS, the 1.56 kb coding region, and 0.44 kb downstream of DHFR-TS.
All but 100 bp of the coding sequence is in a 1.9 kb SalI/EcoRI fragment.
Classification: KINGDOM: Protozoa
References:

Reche P, et al. Cloning and expression of the dihydrofolate reductase-thymidylate synthase gene from Trypanosoma cruzi. Mol. Biochem. Parasitol. 65: 247-258, 1994. PubMed: 7969266

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