yIgH6c2
yIgH6c2
规格:
货期:
编号:TS147501
品牌:Testobio
| 产品名称： | yIgH6c2 |
|---|---|
| 商品货号： | TS147501 |
| Designations： | yIgH6c2 |
| GenBank Number： | Z27510 |
| Species： | Homo sapiens, human |
| Depositors： | HC Riethman |
| Vector： | Construct size (kb): 48.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: SuperCos 1 Intact vector size: 7.600 Type of vector: cosmid Vector end: BamHI Vector end: BamHI Cloning sites: BamHI Polylinker sites: EcoRI NotI BamHI NotI EcoRI Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): marker(s): ampR, neoR, G418R promoter: T3, T7 replicon: pMB1, SV40 Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: D14S308 Gene name: DNA Segment, single copy Contains complete coding sequence?: U Chromosome: 14; Localization: 14 q32.3 Genome: Homo sapiens Gene symbol: IGHV@ Genomic copy number: unique Gene name: immunoglobulin heavy polypeptide, variable region (cluster) Contains complete coding sequence?: U Chromosome: 14; Localization: 14 q32.33 Tissue: leukocyte Type of DNA: genomic Insert end: Sau3AI Insert end: Sau3AI Insert size (kb): 40.0 Cross references: DNA Seq. Acc.: Z27510 DNA Seq. Acc.: Z30083 Insert lengths(kb): 40.0 Tissue: leukocyte Gene product: immunoglobulin heavy-chain variable region IGHV@ |
| Insert Size (kb)： | 40.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: frozen |
| Comments： | IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Contains the 8.5 kb EcoRI fragment encoding V(H) 5-1. Subcloned from yRM2006 (ATCC 99425). One of a set of 2 cosmid contigs ordered, from the telomeric side, yIgH6c10, 2, 4, 9, 5, 14, 3, 19, 1, and 18. The gap is between 9 and 5. These cosmids have been assigned ATCC numbers 99460, 99452, 99456, 99458, 99472, 99462, 99454, 99466, 99450, and 99464, respectively. |
| References： | Cook GP, et al. A map of the human immunoglobulin VH locus completed by analysis of the telomeric region of chromosome 14q. Nat. Genet. 7: 162-168, 1994. PubMed: 7920635 |
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| 产品名称： | yIgH6c2 |
|---|---|
| 商品货号： | TS147501 |
| Designations： | yIgH6c2 |
| GenBank Number： | Z27510 |
| Species： | Homo sapiens, human |
| Depositors： | HC Riethman |
| Vector： | Construct size (kb): 48.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: SuperCos 1 Intact vector size: 7.600 Type of vector: cosmid Vector end: BamHI Vector end: BamHI Cloning sites: BamHI Polylinker sites: EcoRI NotI BamHI NotI EcoRI Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): marker(s): ampR, neoR, G418R promoter: T3, T7 replicon: pMB1, SV40 Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: D14S308 Gene name: DNA Segment, single copy Contains complete coding sequence?: U Chromosome: 14; Localization: 14 q32.3 Genome: Homo sapiens Gene symbol: IGHV@ Genomic copy number: unique Gene name: immunoglobulin heavy polypeptide, variable region (cluster) Contains complete coding sequence?: U Chromosome: 14; Localization: 14 q32.33 Tissue: leukocyte Type of DNA: genomic Insert end: Sau3AI Insert end: Sau3AI Insert size (kb): 40.0 Cross references: DNA Seq. Acc.: Z27510 DNA Seq. Acc.: Z30083 Insert lengths(kb): 40.0 Tissue: leukocyte Gene product: immunoglobulin heavy-chain variable region IGHV@ |
| Insert Size (kb)： | 40.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: frozen |
| Comments： | IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Contains the 8.5 kb EcoRI fragment encoding V(H) 5-1. Subcloned from yRM2006 (ATCC 99425). One of a set of 2 cosmid contigs ordered, from the telomeric side, yIgH6c10, 2, 4, 9, 5, 14, 3, 19, 1, and 18. The gap is between 9 and 5. These cosmids have been assigned ATCC numbers 99460, 99452, 99456, 99458, 99472, 99462, 99454, 99466, 99450, and 99464, respectively. |
| References： | Cook GP, et al. A map of the human immunoglobulin VH locus completed by analysis of the telomeric region of chromosome 14q. Nat. Genet. 7: 162-168, 1994. PubMed: 7920635 |
