| 产品名称： | Vaccinia virus ts mutant |
|---|---|
| 商品货号： | TS147671 |
| Classification： | Poxviridae, Orthopoxvirus |
| Agent： | Vaccinia virus ts mutant |
| Strain： | IHD-W Dts93 (Dales isolate 9281) |
| Applications： | The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U5. |
| Positive for Mycoplasma： | No |
| Biosafety Level： | 2 CDC BMBL recommends vaccination. ref
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | derived from existing strain |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | Selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. The non-permissive incubation temperature is 39.5oC. This mutant was not assigned to a Dales EM category. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U5. |
| Effect on Host： | Yes, Cytopathic effect: Rounding and sloughing of infected cells CPE: enlargement, plaques with rounding and eventual sloughing |
| Recommended Host： | Recommended Host: BSC-40 (ATCC CRL-2761 |
| Growth Conditions： | Temperature: 31.0°C Duration: 1-3 days; For best results, cells should be 24 to 48 hours old and 80-90% confluent at time of infection not 100% confluent. |
| Name of Depositor： | S Dales, RC Condit, S Dales |
| Special Collection： | Orthopoxvirus |
| Source： | derived from existing strain |
| References： | Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807 Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746 This mutant strain was derived by Samuel Dales from the IHD-W strain of Vaccinia (ATCC VR-1441) which in turn was derived from the IHD-J strain. cell enlargement, plaques with rounding and eventual sloughing of cells Biosafety in Microbiological and Biomedical Laboratories 4th ed.; U.S. Department of Health and Human Services ;1999. Non-permissive incubation temperature is 39.5C. This mutant was not assigned to a Dales EM category. It is assigned the Condit map location U5. This subtype is distinguishable from the parent IHD-J strain by its ability to elicit polykaryocytosis in all mammalian and avian cells tested and by its inability to induce an active hemagglutinin at the plasma membrane Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570 |
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | Vaccinia virus ts mutant |
|---|---|
| 商品货号： | TS147671 |
| Classification： | Poxviridae, Orthopoxvirus |
| Agent： | Vaccinia virus ts mutant |
| Strain： | IHD-W Dts93 (Dales isolate 9281) |
| Applications： | The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U5. |
| Positive for Mycoplasma： | No |
| Biosafety Level： | 2 CDC BMBL recommends vaccination. ref
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | derived from existing strain |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | Selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. The non-permissive incubation temperature is 39.5oC. This mutant was not assigned to a Dales EM category. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U5. |
| Effect on Host： | Yes, Cytopathic effect: Rounding and sloughing of infected cells CPE: enlargement, plaques with rounding and eventual sloughing |
| Recommended Host： | Recommended Host: BSC-40 (ATCC CRL-2761 |
| Growth Conditions： | Temperature: 31.0°C Duration: 1-3 days; For best results, cells should be 24 to 48 hours old and 80-90% confluent at time of infection not 100% confluent. |
| Name of Depositor： | S Dales, RC Condit, S Dales |
| Special Collection： | Orthopoxvirus |
| Source： | derived from existing strain |
| References： | Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807 Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746 This mutant strain was derived by Samuel Dales from the IHD-W strain of Vaccinia (ATCC VR-1441) which in turn was derived from the IHD-J strain. cell enlargement, plaques with rounding and eventual sloughing of cells Biosafety in Microbiological and Biomedical Laboratories 4th ed.; U.S. Department of Health and Human Services ;1999. Non-permissive incubation temperature is 39.5C. This mutant was not assigned to a Dales EM category. It is assigned the Condit map location U5. This subtype is distinguishable from the parent IHD-J strain by its ability to elicit polykaryocytosis in all mammalian and avian cells tested and by its inability to induce an active hemagglutinin at the plasma membrane Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570 |
