pUN20 [pNN331]
pUN20 [pNN331]
规格:
货期:
编号:TS147687
品牌:Testobio
| 产品名称： | pUN20 pNN331 |
|---|---|
| 商品货号： | TS147687 |
| Designations： | pUN20 pNN331 |
| Depositors： | SJ Elledge |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.9000000953674320 Vector: pUN20 (plasmid) Promoters: Promoter lac Construction: pUC18 Marker(s):TRP1,ampR,SUP11 Construct size (kb): 5.900000095367432 Features: insert detection: lacZ marker(s): SUP11 marker(s): TRP1 marker(s): ampR promoter: lac replicon: ARS1 replicon: pMB1 MCS: EcoRI...HindIII centromere: CEN4 |
| Applications： | YC-type (centromeric) shuttle vector mutation detection shuttle vector vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.9; KpnI--4.9, 1.0; BamHI--5.9. SUP11 in the vector suppresses the red colony phenotype of ade2-101 ochre allele host strains, grown on limiting adenine media. In the absence of plasmid selection, plasmids are lost at a frequency of 1 to 3%, giving rise to red/white sectoring. One strategy for mutation scanning is to clone the normal allele of a gene into a SUP11 vector and transform into an ade2-101 host with a null mutation in the same gene, thus selecting for plasmid maintenance and producing white colonies. Vectors pUN20 (TS147687), pUN25 (ATCC 77264), pUN60 (ATCC 77266) and pUN65 (ATCC 77267) contain SUP11. A second, potentially mutated copy of the gene can then be cloned into a second centromeric shuttle vector (pUN30, ATCC 77265; pUN70, ATCC 77268; pUN90, ATCC 77269; pUN100, ATCC 77270) for transformation into the SUP11 plasmid containing host. A normal gene in plasmid #2 can relieve the selective pressure on the SUP11 plasmid, restoring the red/white sectoring; a null allele in plasmid #2 will result in SUP11 plasmid maintenance and white colonies. YC-type shuttle vector useful for the sectoring-shuffle mutagenesis assay. Also permits visual detection of recombinants by lacZalpha complementation. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Elledge SJ, Davis RW. A family of versatile centromeric vectors designed for use in the sectoring-shuffle mutagenesis assay in Saccharomyces cerevisiae. Gene 70: 303-312, 1988. PubMed: 3063604 |
| Shipped： | freeze-dried |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
pUN20 [pNN331]
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | pUN20 pNN331 |
|---|---|
| 商品货号： | TS147687 |
| Designations： | pUN20 pNN331 |
| Depositors： | SJ Elledge |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.9000000953674320 Vector: pUN20 (plasmid) Promoters: Promoter lac Construction: pUC18 Marker(s):TRP1,ampR,SUP11 Construct size (kb): 5.900000095367432 Features: insert detection: lacZ marker(s): SUP11 marker(s): TRP1 marker(s): ampR promoter: lac replicon: ARS1 replicon: pMB1 MCS: EcoRI...HindIII centromere: CEN4 |
| Applications： | YC-type (centromeric) shuttle vector mutation detection shuttle vector vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.9; KpnI--4.9, 1.0; BamHI--5.9. SUP11 in the vector suppresses the red colony phenotype of ade2-101 ochre allele host strains, grown on limiting adenine media. In the absence of plasmid selection, plasmids are lost at a frequency of 1 to 3%, giving rise to red/white sectoring. One strategy for mutation scanning is to clone the normal allele of a gene into a SUP11 vector and transform into an ade2-101 host with a null mutation in the same gene, thus selecting for plasmid maintenance and producing white colonies. Vectors pUN20 (TS147687), pUN25 (ATCC 77264), pUN60 (ATCC 77266) and pUN65 (ATCC 77267) contain SUP11. A second, potentially mutated copy of the gene can then be cloned into a second centromeric shuttle vector (pUN30, ATCC 77265; pUN70, ATCC 77268; pUN90, ATCC 77269; pUN100, ATCC 77270) for transformation into the SUP11 plasmid containing host. A normal gene in plasmid #2 can relieve the selective pressure on the SUP11 plasmid, restoring the red/white sectoring; a null allele in plasmid #2 will result in SUP11 plasmid maintenance and white colonies. YC-type shuttle vector useful for the sectoring-shuffle mutagenesis assay. Also permits visual detection of recombinants by lacZalpha complementation. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Elledge SJ, Davis RW. A family of versatile centromeric vectors designed for use in the sectoring-shuffle mutagenesis assay in Saccharomyces cerevisiae. Gene 70: 303-312, 1988. PubMed: 3063604 |
| Shipped： | freeze-dried |
