| 产品名称： | D13-2 |
|---|---|
| 商品货号： | TS147781 |
| Designations： | D13-2 |
| GenBank Number： | M28561 |
| Species： | Mus musculus, mouse |
| Depositors： | DW Sears |
| Applications： | in another host, produces protein Fc gamma high affinity receptor (IgG2a) |
| Vector： | Construct size (kb): 4.311999797821045 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.279000043869019 Tissue: macrophage J774 cell line Gene product: Fc gamma high affinity receptor (IgG2a) Fcr1 Target Gene: Fc gamma high affinity receptor (IgG2a) |
| Insert Size (kb)： | 1.279 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--3.6, 0.6, 0.1; BamHI--3.0, 1.4; BglI--2.8, 1.3, 0.3; HindIII--4.35; SacI--3.8, 0.5. Restriction digest of the clone give the following sizes (kb): PstI--3.6, 0.6; BamHI--3.0, 1.4; BglI--2.8, 1.3, 0.3; HindIII--4.35; SacI--3.8, 0.5. The cDNA detects a single genomic HindIII fragment (8.8 kb), a doublet of EcoRI fragments (4.3 kb) and several TaqI, PvuII, BamHI, and BclI fragments. The insert contains a complete open reading frame of 1212 nucleotides, one nucleotide 5 untranslated (C), and 61 nucleotides 3 untranslated followed by AAAAA. The vector is a derivative of pcD with added restriction sites (BamHI, XmaI SmaI KpnI SacI) and a T3 promoter 3 of the insert site. When excised with BamHI, the insert will contain 5 and 3 vector sequences. The BamHI sites are at positions 414 and 541 of the vector and the PstI site is at 505. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Sears DW, et al. Molecular cloning and expression of the mouse high affinity Fc receptor for IgG1. J. Immunol. 144: 371-378, 1990. PubMed: 2136886 Duane W Sears, personal communication |
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| 产品名称： | D13-2 |
|---|---|
| 商品货号： | TS147781 |
| Designations： | D13-2 |
| GenBank Number： | M28561 |
| Species： | Mus musculus, mouse |
| Depositors： | DW Sears |
| Applications： | in another host, produces protein Fc gamma high affinity receptor (IgG2a) |
| Vector： | Construct size (kb): 4.311999797821045 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.279000043869019 Tissue: macrophage J774 cell line Gene product: Fc gamma high affinity receptor (IgG2a) Fcr1 Target Gene: Fc gamma high affinity receptor (IgG2a) |
| Insert Size (kb)： | 1.279 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--3.6, 0.6, 0.1; BamHI--3.0, 1.4; BglI--2.8, 1.3, 0.3; HindIII--4.35; SacI--3.8, 0.5. Restriction digest of the clone give the following sizes (kb): PstI--3.6, 0.6; BamHI--3.0, 1.4; BglI--2.8, 1.3, 0.3; HindIII--4.35; SacI--3.8, 0.5. The cDNA detects a single genomic HindIII fragment (8.8 kb), a doublet of EcoRI fragments (4.3 kb) and several TaqI, PvuII, BamHI, and BclI fragments. The insert contains a complete open reading frame of 1212 nucleotides, one nucleotide 5 untranslated (C), and 61 nucleotides 3 untranslated followed by AAAAA. The vector is a derivative of pcD with added restriction sites (BamHI, XmaI SmaI KpnI SacI) and a T3 promoter 3 of the insert site. When excised with BamHI, the insert will contain 5 and 3 vector sequences. The BamHI sites are at positions 414 and 541 of the vector and the PstI site is at 505. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Sears DW, et al. Molecular cloning and expression of the mouse high affinity Fc receptor for IgG1. J. Immunol. 144: 371-378, 1990. PubMed: 2136886 Duane W Sears, personal communication |
