| 产品名称： | Betacoronavirus 1 |
|---|---|
| 商品货号： | TS148018 |
| Classification： | Coronaviridae, Betacoronavirus |
| Deposited As： | Human coronavirus OC43 |
| Agent： | Betacoronavirus 1 |
| Strain： | OC43 |
| Common Name： | Human coronavirus OC43 |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | VR-1558™ is a TC-adapted item derived from virus passaged in mice (ATCC® VR-759™).xa0 Cleaned of mycoplasma with MRA.xa0 No antigenic cross reaction with Human coronavirus 229E (ATCC® VR-740™). |
| Effect on Host： | CPE, vacuolization of cytoplasm and sloughing |
| Recommended Host： | HCT-8 (ATCC® CCL-244™) |
| Growth Conditions： | Temperature: 33°C Recommendations for Infection: For best results, infection should be performed on an 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer two times with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 33°C in a humidified 5% CO2 atmosphere, while rocking continuously. End adsorption by adding virus growth medium. Incubation: 4-6 days at 33°C in a humidified 5% CO2 atmosphere, rocking continuously, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | JO Fleming, University of Wisconsin, Madison |
| Special Collection： | ATCC |
| Chain of Custody： | ATCC |
| Source： | Man with cold-like illness. Treated to remove mycoplasma at ATCC. |
| References： | Bruckova M, et al. The adaptation of two human coronavirus strains (OC38 and OC43) to growth in cell monolayers. Proc. Soc. Exp. Biol. Med. 135: 431-435, 1970. PubMed: 4991398 McIntosh K, et al. Growth in suckling-mouse brain of "IBV-like" viruses from patients with upper respiratory tract disease. Proc. Natl. Acad. Sci. USA 58: 2268-2273, 1967. PubMed: 4298953 McIntosh K, et al. Recovery in tracheal organ cultures of novel viruses from patients with respiratory disease. Proc. Natl. Acad. Sci. USA 57: 933-940, 1967. PubMed: 5231356 |
| Cross References： | Nucleotide (GenBank) : NC_005147.1 Human coronavirus OC43, complete genome |
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| 产品名称： | Betacoronavirus 1 |
|---|---|
| 商品货号： | TS148018 |
| Classification： | Coronaviridae, Betacoronavirus |
| Deposited As： | Human coronavirus OC43 |
| Agent： | Betacoronavirus 1 |
| Strain： | OC43 |
| Common Name： | Human coronavirus OC43 |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： | |
| Comments： | VR-1558™ is a TC-adapted item derived from virus passaged in mice (ATCC® VR-759™).xa0 Cleaned of mycoplasma with MRA.xa0 No antigenic cross reaction with Human coronavirus 229E (ATCC® VR-740™). |
| Effect on Host： | CPE, vacuolization of cytoplasm and sloughing |
| Recommended Host： | HCT-8 (ATCC® CCL-244™) |
| Growth Conditions： | Temperature: 33°C Recommendations for Infection: For best results, infection should be performed on an 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer two times with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 33°C in a humidified 5% CO2 atmosphere, while rocking continuously. End adsorption by adding virus growth medium. Incubation: 4-6 days at 33°C in a humidified 5% CO2 atmosphere, rocking continuously, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | JO Fleming, University of Wisconsin, Madison |
| Special Collection： | ATCC |
| Chain of Custody： | ATCC |
| Source： | Man with cold-like illness. Treated to remove mycoplasma at ATCC. |
| References： | Bruckova M, et al. The adaptation of two human coronavirus strains (OC38 and OC43) to growth in cell monolayers. Proc. Soc. Exp. Biol. Med. 135: 431-435, 1970. PubMed: 4991398 McIntosh K, et al. Growth in suckling-mouse brain of "IBV-like" viruses from patients with upper respiratory tract disease. Proc. Natl. Acad. Sci. USA 58: 2268-2273, 1967. PubMed: 4298953 McIntosh K, et al. Recovery in tracheal organ cultures of novel viruses from patients with respiratory disease. Proc. Natl. Acad. Sci. USA 57: 933-940, 1967. PubMed: 5231356 |
| Cross References： | Nucleotide (GenBank) : NC_005147.1 Human coronavirus OC43, complete genome |
