D2a-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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D2a
D2a
规格:
货期:
编号:TS148445
品牌:Testobio
产品名称: D2a
商品货号: TS148445
Designations: D2a
GenBank Number:

L10717

Species: Homo sapiens, human
Applications:
A 2.8 kb PstI fragment from the unique and untranslated 5 domain has been used as a probe.
Vector:
Construct size (kb): 12.39999961853027
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript KS
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: SacI BstXI SacII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV
HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI
Polylinker sites: SEE COMMENTS
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ITK
Genomic copy number: unique
Gene name: T-cell-specific tyrosine kinase; homolog of mouse T-cell
itk/tsk tyrosine kinase
Contains complete coding sequence?: Y
Chromosome: 5; Localization: 5 q31-q32
Tissue: thymus
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 9.4
Cross references: DNA Seq. Acc.: L10717
Insert lengths(kb): 9.399999618530273
Tissue: thymus
Gene product: T-cell-specific tyrosine kinase; homolog of mouse T-cell itk/tsk tyrosine kinase ITK
Insert Size (kb): 9.400
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): BamHI--6.5, 3.6, 2.5; EcoRI--4.8, 3.1, 2.5, 2.0; PstI--9.2, 2.8, 0.56; XbaI--5.9, 3.8, 1.8, 0.7; HindIII--4.5, 4.3, 2.5, 1.1.
The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--5.92; BglI--2.13; BglII--4.67; EcoRI--1.95; EcoRV--4.48; HindIII--1.32, 2.43; KpnI--1.90; PstI--2.83, 3.39; SstI--2.19, 3.11; XbaI--5.26, 5.95.
Contains the complete coding sequence (nt 2024-3886).
A 2.8 kb PstI fragment from the unique and untranslated 5 domain has been used as a probe.
References:

Gibson S, et al. Identification, cloning, and characterization of a novel human T-cell-specific tyrosine kinase located at the hematopoietin complex on chromosome 5q. Blood 82: 1561-1572, 1993. PubMed: 8364206

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D2a

  • 货号: TS148445
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: D2a
商品货号: TS148445
Designations: D2a
GenBank Number:

L10717

Species: Homo sapiens, human
Applications:
A 2.8 kb PstI fragment from the unique and untranslated 5 domain has been used as a probe.
Vector:
Construct size (kb): 12.39999961853027
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript KS
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: SacI BstXI SacII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV
HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI
Polylinker sites: SEE COMMENTS
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ITK
Genomic copy number: unique
Gene name: T-cell-specific tyrosine kinase; homolog of mouse T-cell
itk/tsk tyrosine kinase
Contains complete coding sequence?: Y
Chromosome: 5; Localization: 5 q31-q32
Tissue: thymus
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 9.4
Cross references: DNA Seq. Acc.: L10717
Insert lengths(kb): 9.399999618530273
Tissue: thymus
Gene product: T-cell-specific tyrosine kinase; homolog of mouse T-cell itk/tsk tyrosine kinase ITK
Insert Size (kb): 9.400
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): BamHI--6.5, 3.6, 2.5; EcoRI--4.8, 3.1, 2.5, 2.0; PstI--9.2, 2.8, 0.56; XbaI--5.9, 3.8, 1.8, 0.7; HindIII--4.5, 4.3, 2.5, 1.1.
The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--5.92; BglI--2.13; BglII--4.67; EcoRI--1.95; EcoRV--4.48; HindIII--1.32, 2.43; KpnI--1.90; PstI--2.83, 3.39; SstI--2.19, 3.11; XbaI--5.26, 5.95.
Contains the complete coding sequence (nt 2024-3886).
A 2.8 kb PstI fragment from the unique and untranslated 5 domain has been used as a probe.
References:

Gibson S, et al. Identification, cloning, and characterization of a novel human T-cell-specific tyrosine kinase located at the hematopoietin complex on chromosome 5q. Blood 82: 1561-1572, 1993. PubMed: 8364206

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