| 产品名称： | alpha1JN alphaNJ, pJN I |
|---|---|
| 商品货号： | TS148498 |
| Designations： | alpha1JN alphaNJ, pJN I |
| GenBank Number： | J00067 |
| Species： | Homo sapiens, human |
| Depositors： | NA Kalsheker |
| Applications： | Enzyme(s) not detecting polymorphism (for alpha1-antitrypsin gene-related sequence): BamHI, HindIII, MspI, PstI, XbaI, XhoII (tested against 20 unrelated Caucasians). |
| Vector： | Construct size (kb): 4.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.25 Tissue: liver Gene product: alpha-1-antitrypsin-like PI Alleles: G3, A1, A2, B1, B2, D2, G1, G2, J2, N1, N2, C1, C2, D1, J1, A1, A2, D1, D2 |
| Insert Size (kb)： | 0.25 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/AvaI--2.55, 1.45; PstI--4.0; EcoRI--4.0; AvaI--4.0; HindIII--4.0. The sequence starts at amino acid 363 and continues through a poly(A) tail of 50 bp. The PstI site at the 3 end was not reconstituted. The insert encompasses the last exon including the 3 untranslated region. Codominant segregation for the EcoRI RFLP has been demonstrated in 2 informative families. The gene-related sequence which demonstrates the EcoRI polymorphism maps 15 kb downstream from the alpha-1-antitrypsin gene. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. The clone is for alpha1-antitrypsin but can detect a related sequence. Signals can be distinguished by the sequence and the sizes of fragments generated. At higher stringency this can be made specific for alpha1-antitrypsin. Enzyme(s) not detecting polymorphism (for alpha1-antitrypsin gene-related sequence): BamHI, HindIII, MspI, PstI, XbaI, XhoII (tested against 20 unrelated Caucasians). |
| References： | Rogers J, et al. The isolation of a clone for human alpha 1-antitrypsin and the detection of alpha 1-antitrypsin in mRNA from liver and leukocytes. Biochem. Biophys. Res. Commun. 116: 375-382, 1983. PubMed: 6606425 Hodgson I, Kalsheker N. RFLP for a gene-related sequence of alpha 1-antitrypsin (AAT). Nucleic Acids Res. 14: 6779, 1986. PubMed: 3018681 Barbara Weiffenbach, personal communication Noor A Kalsheker, personal communication |
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| 产品名称： | alpha1JN alphaNJ, pJN I |
|---|---|
| 商品货号： | TS148498 |
| Designations： | alpha1JN alphaNJ, pJN I |
| GenBank Number： | J00067 |
| Species： | Homo sapiens, human |
| Depositors： | NA Kalsheker |
| Applications： | Enzyme(s) not detecting polymorphism (for alpha1-antitrypsin gene-related sequence): BamHI, HindIII, MspI, PstI, XbaI, XhoII (tested against 20 unrelated Caucasians). |
| Vector： | Construct size (kb): 4.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.25 Tissue: liver Gene product: alpha-1-antitrypsin-like PI Alleles: G3, A1, A2, B1, B2, D2, G1, G2, J2, N1, N2, C1, C2, D1, J1, A1, A2, D1, D2 |
| Insert Size (kb)： | 0.25 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/AvaI--2.55, 1.45; PstI--4.0; EcoRI--4.0; AvaI--4.0; HindIII--4.0. The sequence starts at amino acid 363 and continues through a poly(A) tail of 50 bp. The PstI site at the 3 end was not reconstituted. The insert encompasses the last exon including the 3 untranslated region. Codominant segregation for the EcoRI RFLP has been demonstrated in 2 informative families. The gene-related sequence which demonstrates the EcoRI polymorphism maps 15 kb downstream from the alpha-1-antitrypsin gene. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. The clone is for alpha1-antitrypsin but can detect a related sequence. Signals can be distinguished by the sequence and the sizes of fragments generated. At higher stringency this can be made specific for alpha1-antitrypsin. Enzyme(s) not detecting polymorphism (for alpha1-antitrypsin gene-related sequence): BamHI, HindIII, MspI, PstI, XbaI, XhoII (tested against 20 unrelated Caucasians). |
| References： | Rogers J, et al. The isolation of a clone for human alpha 1-antitrypsin and the detection of alpha 1-antitrypsin in mRNA from liver and leukocytes. Biochem. Biophys. Res. Commun. 116: 375-382, 1983. PubMed: 6606425 Hodgson I, Kalsheker N. RFLP for a gene-related sequence of alpha 1-antitrypsin (AAT). Nucleic Acids Res. 14: 6779, 1986. PubMed: 3018681 Barbara Weiffenbach, personal communication Noor A Kalsheker, personal communication |
