phgH6 [hGH6]-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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phgH6 [hGH6]
phgH6 [hGH6]
规格:
货期:
编号:TS148569
品牌:Testobio
产品名称: phgH6 hGH6
商品货号: TS148569
Designations: phgH6 hGH6
GenBank Number:

X13461

Species: Homo sapiens, human
Vector:
Construct size (kb): 5.400000095367432
Insert:
DNA: genomic
Insert lengths(kb): 2.733999967575073
Tissue: leukocyte
Gene product: calmodulin-like CALML3
Insert Size (kb): 2.734
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.3, 1.15; PvuII--2.4, 1.1, 0.95, 0.75, 0.35; SacI--2.7 (doublet); SacI/BglI--1.4, 1.15, 0.9, 0.85, 0.65, 0.4; SmaI--3.7, 1.2, 0.75; SmaI/SacI--2.7, 1.2, 0.9, 0.75.
The insert contains the following restriction sites (nt from the 5 end): PstI--106, 752, 1741, 1905, 1998, 2208; PvuII--162, 1077, 2144; SmaI--863, 1569; NotI--953; EcoRI--1099.
Contains a complete open reading frame of 444 nucleotides, with 5 and 3 flanking sequences.
A 0.7 kb SmaI fragment containing the coding region (nt 863 -1569) detects a 2.3 kb mRNA in human erythroleukemia K562 cells. A 1.2 kb SmaI/SacI fragment from the 3 region (nt 1570 - 2738) detects a 1.5 kb mRNA in SV40-transformed human fibroblasts.
The intact insert can be separated from the vector by a SacI/SmaI double digest.
References:

Koller M, Strehler EE. Characterization of an intronless human calmodulin-like pseudogene. FEBS Lett. 239: 121-128, 1988. PubMed: 3181418

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phgH6 [hGH6]

  • 货号: TS148569
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: phgH6 hGH6
商品货号: TS148569
Designations: phgH6 hGH6
GenBank Number:

X13461

Species: Homo sapiens, human
Vector:
Construct size (kb): 5.400000095367432
Insert:
DNA: genomic
Insert lengths(kb): 2.733999967575073
Tissue: leukocyte
Gene product: calmodulin-like CALML3
Insert Size (kb): 2.734
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.3, 1.15; PvuII--2.4, 1.1, 0.95, 0.75, 0.35; SacI--2.7 (doublet); SacI/BglI--1.4, 1.15, 0.9, 0.85, 0.65, 0.4; SmaI--3.7, 1.2, 0.75; SmaI/SacI--2.7, 1.2, 0.9, 0.75.
The insert contains the following restriction sites (nt from the 5 end): PstI--106, 752, 1741, 1905, 1998, 2208; PvuII--162, 1077, 2144; SmaI--863, 1569; NotI--953; EcoRI--1099.
Contains a complete open reading frame of 444 nucleotides, with 5 and 3 flanking sequences.
A 0.7 kb SmaI fragment containing the coding region (nt 863 -1569) detects a 2.3 kb mRNA in human erythroleukemia K562 cells. A 1.2 kb SmaI/SacI fragment from the 3 region (nt 1570 - 2738) detects a 1.5 kb mRNA in SV40-transformed human fibroblasts.
The intact insert can be separated from the vector by a SacI/SmaI double digest.
References:

Koller M, Strehler EE. Characterization of an intronless human calmodulin-like pseudogene. FEBS Lett. 239: 121-128, 1988. PubMed: 3181418

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