| 产品名称： | HD2G1 |
|---|---|
| 商品货号： | TS148905 |
| Designations： | HD2G1 |
| GenBank Number： | M29066 |
| Species： | Homo sapiens, human |
| Depositors： | O Civelli, DK Grandy |
| Applications： | Codominant inheritance was observed in 3 informative families (39 children). |
| Vector： | Construct size (kb): 44.0 |
| Insert： | DNA: genomic Insert lengths(kb): 16.0 Tissue: male Gene product: dopamine receptor D2 DRD2 Alleles: A1, B1, B2, A2 |
| Insert Size (kb)： | 16.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried bacteria-free lysate |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--20.0, 9.0, 5.0, 4.3, 1.8, 1.7, 1.7, 0.7; BglII--25.0, 7.0, 3.2, 3.0, 2.4, 1.5, 0.68; PvuI--20.0, 12.0, 8.0; HindIII--21.0, 18.0, 4.3, 0.8; SmaI--20.0, 19.0, 5.6. The insert contains the following sites separated by (kb)(approx): SalI, MboII- 1.5- BamHI- 1.7- BamHI- 1.7- BamHI- 4.3- BamHI- 1.8- BamHI- 0.65- BamHI- 5.0- SmaI- 0.6- BamHI- 0.7- MboII, SalI. Codominant inheritance was observed in 3 informative families (39 children). The 1.6 kb BamHI fragment contains exon 7 and 3 sequences. Exon 7, which has been sequenced, encodes the last 64 amino acids and includes 1.2 kb of 3 non-coding sequence. The BamHI 1.6 doublet detects the TaqI RFLP associated with DRD2. Use standard hybridization conditions with the addition of human placental DNA to block repeat sequences. Washing conditions are standard with a final wash at 0.1 x SSC, 0.1% SDS, 65C. Enzyme(s) not detecting polymorphism: 31 enzymes. |
| References： | Grandy DK, et al. The human dopamine D2 receptor gene is located on chromosome 11 at q22-q23 and identifies a TaqI RFLP. Am. J. Hum. Genet. 45: 778-785, 1989. PubMed: 2573278 Grandy DK, et al. Cloning of the cDNA and gene for a human D2 dopamine receptor. Proc. Natl. Acad. Sci. USA 86: 9762-9766, 1989. PubMed: 2532362 Olivier Civelli, personal communication |
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| 产品名称： | HD2G1 |
|---|---|
| 商品货号： | TS148905 |
| Designations： | HD2G1 |
| GenBank Number： | M29066 |
| Species： | Homo sapiens, human |
| Depositors： | O Civelli, DK Grandy |
| Applications： | Codominant inheritance was observed in 3 informative families (39 children). |
| Vector： | Construct size (kb): 44.0 |
| Insert： | DNA: genomic Insert lengths(kb): 16.0 Tissue: male Gene product: dopamine receptor D2 DRD2 Alleles: A1, B1, B2, A2 |
| Insert Size (kb)： | 16.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried bacteria-free lysate |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--20.0, 9.0, 5.0, 4.3, 1.8, 1.7, 1.7, 0.7; BglII--25.0, 7.0, 3.2, 3.0, 2.4, 1.5, 0.68; PvuI--20.0, 12.0, 8.0; HindIII--21.0, 18.0, 4.3, 0.8; SmaI--20.0, 19.0, 5.6. The insert contains the following sites separated by (kb)(approx): SalI, MboII- 1.5- BamHI- 1.7- BamHI- 1.7- BamHI- 4.3- BamHI- 1.8- BamHI- 0.65- BamHI- 5.0- SmaI- 0.6- BamHI- 0.7- MboII, SalI. Codominant inheritance was observed in 3 informative families (39 children). The 1.6 kb BamHI fragment contains exon 7 and 3 sequences. Exon 7, which has been sequenced, encodes the last 64 amino acids and includes 1.2 kb of 3 non-coding sequence. The BamHI 1.6 doublet detects the TaqI RFLP associated with DRD2. Use standard hybridization conditions with the addition of human placental DNA to block repeat sequences. Washing conditions are standard with a final wash at 0.1 x SSC, 0.1% SDS, 65C. Enzyme(s) not detecting polymorphism: 31 enzymes. |
| References： | Grandy DK, et al. The human dopamine D2 receptor gene is located on chromosome 11 at q22-q23 and identifies a TaqI RFLP. Am. J. Hum. Genet. 45: 778-785, 1989. PubMed: 2573278 Grandy DK, et al. Cloning of the cDNA and gene for a human D2 dopamine receptor. Proc. Natl. Acad. Sci. USA 86: 9762-9766, 1989. PubMed: 2532362 Olivier Civelli, personal communication |
