pBS-human p57 [KIP2]-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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pBS-human p57 [KIP2]
pBS-human p57 [KIP2]
规格:
货期:
编号:TS148924
品牌:Testobio
产品名称: pBS-human p57 KIP2
商品货号: TS148924
Designations: pBS-human p57 KIP2
GenBank Number:

U22398

Species: Homo sapiens, human
Vector:
Construct size (kb): 4.460000038146973
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II KS+
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ
marker(s): ampR
replicon: pMB1, f1
Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CDKN1C
Other gene symbols: KIP2
Gene name: cyclin-dependent kinase inhibitor 1C (p57, Kip2)
Contains complete coding sequence?: Y
Chromosome: 11; Localization: 11 p15.5
Tissue: embryo
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 1.5
Cross references: DNA Seq. Acc.: U22398
Nucleotides 1-1511 of the insert correspond to
nucleotides 1-1511 of U22398.
Insert lengths(kb): 1.5
Tissue: embryo
Gene product: cyclin-dependent kinase inhibitor 1C (p57, Kip2)(P57, kinase inhibitory protein 2 of cyclins (p57)) KIP2
Insert Size (kb): 1.5
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): BamHI--4.6; EcoRI--3.0, 1.6; EcoRI/HindIII--3.0, 1.55; EcoRI/PstI--3.0, 1.15, 0.47; PstI--3.5, 1.15; SmaI--3.2, 1.4.
The insert contains the following restriction sites (approximate kb from the 5 end): PstI--0.4, 0.49; HindIII--1.44; SmaI--0.25.
References:

Matsuoka S, et al. p57KIP2, a structurally distinct member of the p21CIP1 Cdk-inhibitor family, is a candidate tumor suppressor gene. Genes Dev. 9: 650-662, 1995. PubMed: 7729684

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pBS-human p57 [KIP2]

  • 货号: TS148924
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: pBS-human p57 KIP2
商品货号: TS148924
Designations: pBS-human p57 KIP2
GenBank Number:

U22398

Species: Homo sapiens, human
Vector:
Construct size (kb): 4.460000038146973
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II KS+
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ
marker(s): ampR
replicon: pMB1, f1
Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CDKN1C
Other gene symbols: KIP2
Gene name: cyclin-dependent kinase inhibitor 1C (p57, Kip2)
Contains complete coding sequence?: Y
Chromosome: 11; Localization: 11 p15.5
Tissue: embryo
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 1.5
Cross references: DNA Seq. Acc.: U22398
Nucleotides 1-1511 of the insert correspond to
nucleotides 1-1511 of U22398.
Insert lengths(kb): 1.5
Tissue: embryo
Gene product: cyclin-dependent kinase inhibitor 1C (p57, Kip2)(P57, kinase inhibitory protein 2 of cyclins (p57)) KIP2
Insert Size (kb): 1.5
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): BamHI--4.6; EcoRI--3.0, 1.6; EcoRI/HindIII--3.0, 1.55; EcoRI/PstI--3.0, 1.15, 0.47; PstI--3.5, 1.15; SmaI--3.2, 1.4.
The insert contains the following restriction sites (approximate kb from the 5 end): PstI--0.4, 0.49; HindIII--1.44; SmaI--0.25.
References:

Matsuoka S, et al. p57KIP2, a structurally distinct member of the p21CIP1 Cdk-inhibitor family, is a candidate tumor suppressor gene. Genes Dev. 9: 650-662, 1995. PubMed: 7729684

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