| 产品名称： | UACC-462 |
|---|---|
| 商品货号： | TS148933 |
| Organism： | Homo sapiens, human |
| Tissue： | pancreas; derived from metastatic site: peritoneal mass |
| Cell Type： | epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | pancreatic cancer |
| Age： | 41 |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | UACC-462 cell line was derived from a 41 year-old male with pancreatic cancer metastatic to the peritoneal mass. |
| Clinical Data： | 41 years male Caucasian |
| Complete Growth Medium： | M-41 medium. The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium (final conc.):
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of solutions for culture vessels of other sizes. 1. Remove and discard culture medium. 2. Briefly rinse the cell layer with 5.0 ml of Ca++/Mg++ free Dulbeccos phosphate-buffered saline (DPBS) to remove all traces of serum which contains trypsin inhibitor. 3. Add 5.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 4. Add 5.0 ml of complete growth medium and aspirate cells by gently pipetting. 5. Transfer all cell suspension to a 15ml centrifuge tube and spin at approximately 125 x g for 10 minutes. Discard supernatant.
6. Re-suspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.xa0 |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 10% (v/v) FBS and 10% (v/v) DMSO.xa0
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 100%.
Temperature: 37°C |
| Cells per Vial： | ≥ 1.0 x 10^6 |
| STR Profile： | TH01: 9, 9.3 D5S818: 12, 13 D13S317: xa09, 12 D7S820: 12 D16S539: 13 CSF1PO: 10, 12 Amelogenin: X, Y vWA: 17 TPOX: 8, 10 |
| Name of Depositor： | Kathy Brown |
| Passage History： | 38 |
| Year of Origin： | 1985 |
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| 产品名称： | UACC-462 |
|---|---|
| 商品货号： | TS148933 |
| Organism： | Homo sapiens, human |
| Tissue： | pancreas; derived from metastatic site: peritoneal mass |
| Cell Type： | epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | pancreatic cancer |
| Age： | 41 |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： | |
| Derivation： | UACC-462 cell line was derived from a 41 year-old male with pancreatic cancer metastatic to the peritoneal mass. |
| Clinical Data： | 41 years male Caucasian |
| Complete Growth Medium： | M-41 medium. The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium (final conc.):
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of solutions for culture vessels of other sizes. 1. Remove and discard culture medium. 2. Briefly rinse the cell layer with 5.0 ml of Ca++/Mg++ free Dulbeccos phosphate-buffered saline (DPBS) to remove all traces of serum which contains trypsin inhibitor. 3. Add 5.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 4. Add 5.0 ml of complete growth medium and aspirate cells by gently pipetting. 5. Transfer all cell suspension to a 15ml centrifuge tube and spin at approximately 125 x g for 10 minutes. Discard supernatant.
6. Re-suspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.xa0 |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 10% (v/v) FBS and 10% (v/v) DMSO.xa0
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 100%.
Temperature: 37°C |
| Cells per Vial： | ≥ 1.0 x 10^6 |
| STR Profile： | TH01: 9, 9.3 D5S818: 12, 13 D13S317: xa09, 12 D7S820: 12 D16S539: 13 CSF1PO: 10, 12 Amelogenin: X, Y vWA: 17 TPOX: 8, 10 |
| Name of Depositor： | Kathy Brown |
| Passage History： | 38 |
| Year of Origin： | 1985 |
