| 产品名称： | C1-1/5 D7S2742, UTR-9783 |
|---|---|
| 商品货号： | TS148947 |
| Designations： | C1-1/5 D7S2742, UTR-9783 |
| GenBank Number： | M28668 |
| Species： | Homo sapiens, human |
| Depositors： | JR Riordan |
| Vector： | Vector: pBluescript SK- (phagemid) Size (kb): 2.960 Construction: pUC19 Marker(s): ampR (50 ug/ml) Features: Insert detection: lacZ; Promoters: T3, T7, lac; Replicons: f1, pMB1; MCS: KpnI...SacI Sequencing primers: M13(-21), M13 reverse, T7, T3 |
| Insert： | DNA: cDNA
Insert lengths(kb): 3.25
Tissue: cystic fibrosis sweat gland
Gene product: cystic fibrosis transmembrane conductance regulator CF
Alleles: A1, A1, A1, A1, A2, B1, B2, A1, A1, A2, B1, B2, A2, A2, A2, A2 |
| Insert Size (kb)： | 3.25 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Contains 2 internal EcoRI sites. An EcoRI digest of the plasmid results in fragments of 1.0, 1.5, and 0.75 kb from the insert. Restriction digests of the clone give the following sizes (kb): EcoRI--2.65, 1.5, 1.1, 0.89; XbaI--6.2; PstI--6.2; BglII--5.7, 0.45; HindIII--3.2, 1.85, 0.94. TS148947 has been converted to meet the requirements of the Budapest Treaty for patent deposits. A compliance form is not needed for TS148947, but is for ATCC 61160. This probe contains a 3 bp deletion (deltaF508) in exon 10. This clone includes exons 9 - 24 and hybridizes to genomic EcoRI fragments of 10.3, 4.3, 3.5, 2.4, 0.5, 1.8, 1.8, 3.5, 1.9, 4.5, 2.4, 1.0. 2.8, 1.8, 8.5, 2.2, and 2.3 kb. |
| References： | Riordan JR, et al. Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA. Science 245: 1066-1073, 1989. PubMed: 2475911 J R Riordan, personal communication |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | C1-1/5 D7S2742, UTR-9783 |
|---|---|
| 商品货号： | TS148947 |
| Designations： | C1-1/5 D7S2742, UTR-9783 |
| GenBank Number： | M28668 |
| Species： | Homo sapiens, human |
| Depositors： | JR Riordan |
| Vector： | Vector: pBluescript SK- (phagemid) Size (kb): 2.960 Construction: pUC19 Marker(s): ampR (50 ug/ml) Features: Insert detection: lacZ; Promoters: T3, T7, lac; Replicons: f1, pMB1; MCS: KpnI...SacI Sequencing primers: M13(-21), M13 reverse, T7, T3 |
| Insert： | DNA: cDNA
Insert lengths(kb): 3.25
Tissue: cystic fibrosis sweat gland
Gene product: cystic fibrosis transmembrane conductance regulator CF
Alleles: A1, A1, A1, A1, A2, B1, B2, A1, A1, A2, B1, B2, A2, A2, A2, A2 |
| Insert Size (kb)： | 3.25 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Contains 2 internal EcoRI sites. An EcoRI digest of the plasmid results in fragments of 1.0, 1.5, and 0.75 kb from the insert. Restriction digests of the clone give the following sizes (kb): EcoRI--2.65, 1.5, 1.1, 0.89; XbaI--6.2; PstI--6.2; BglII--5.7, 0.45; HindIII--3.2, 1.85, 0.94. TS148947 has been converted to meet the requirements of the Budapest Treaty for patent deposits. A compliance form is not needed for TS148947, but is for ATCC 61160. This probe contains a 3 bp deletion (deltaF508) in exon 10. This clone includes exons 9 - 24 and hybridizes to genomic EcoRI fragments of 10.3, 4.3, 3.5, 2.4, 0.5, 1.8, 1.8, 3.5, 1.9, 4.5, 2.4, 1.0. 2.8, 1.8, 8.5, 2.2, and 2.3 kb. |
| References： | Riordan JR, et al. Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA. Science 245: 1066-1073, 1989. PubMed: 2475911 J R Riordan, personal communication |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
