| 产品名称： | p52 c- p52C- plasmid in E. coli |
|---|---|
| 商品货号： | TS149059 |
| Designations： | p52 c- p52C- plasmid in E. coli |
| Species： | Homo sapiens, human |
| Depositors： | RA Weinberg |
| Applications： | Contains the 3 untranslated region and has been used to detect transcripts. |
| Vector： | Construct size (kb): 4.2 |
| Insert： | DNA: genomic Insert lengths(kb): 1.5 Tissue: HL-60 cell line (promyelocytic leukemia) Gene product: neuroblastoma RAS viral (v-ras) oncogene homolog( v-ras neuroblastoma RAS viral oncogene homolog, proto-oncogene N-ras) NRAS Alleles: A1, A2 |
| Insert Size (kb)： | 1.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--2.85, 1.3; EcoRI--2.7, 1.45. Contains the 3 untranslated region and has been used to detect transcripts. The insert contains AccI and PstI sites. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. EcoRI linkers were added during construction and cloned into EcoRI site of pUC12. |
| References： | Murray MJ, et al. The HL-60 transforming sequence: a ras oncogene coexisting with altered myc genes in hematopoietic tumors. Cell 33: 749-757, 1983. PubMed: 6683594 Barbara Weiffenbach, personal communication |
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| 产品名称： | p52 c- p52C- plasmid in E. coli |
|---|---|
| 商品货号： | TS149059 |
| Designations： | p52 c- p52C- plasmid in E. coli |
| Species： | Homo sapiens, human |
| Depositors： | RA Weinberg |
| Applications： | Contains the 3 untranslated region and has been used to detect transcripts. |
| Vector： | Construct size (kb): 4.2 |
| Insert： | DNA: genomic Insert lengths(kb): 1.5 Tissue: HL-60 cell line (promyelocytic leukemia) Gene product: neuroblastoma RAS viral (v-ras) oncogene homolog( v-ras neuroblastoma RAS viral oncogene homolog, proto-oncogene N-ras) NRAS Alleles: A1, A2 |
| Insert Size (kb)： | 1.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--2.85, 1.3; EcoRI--2.7, 1.45. Contains the 3 untranslated region and has been used to detect transcripts. The insert contains AccI and PstI sites. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. EcoRI linkers were added during construction and cloned into EcoRI site of pUC12. |
| References： | Murray MJ, et al. The HL-60 transforming sequence: a ras oncogene coexisting with altered myc genes in hematopoietic tumors. Cell 33: 749-757, 1983. PubMed: 6683594 Barbara Weiffenbach, personal communication |
