| 产品名称： | pBG311.bmis IVI 10090 |
|---|---|
| 商品货号： | TS149193 |
| Designations： | pBG311.bmis IVI 10090 |
| GenBank Number： | M13151 |
| Species： | Bos taurus, cow |
| Depositors： | Biogen, Inc., RL Cate, Biogen, Inc. |
| Applications： | in another host, produces protein anti-Mullerian hormone (Mullerian inhibiting substance) |
| Vector： | Construct size (kb): 6.535999774932861 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.04200005531311 Tissue: testis Gene product: anti-Mullerian hormone (Mullerian inhibiting substance) AMH Target Gene: anti-Mullerian hormone (Mullerian inhibiting substance) |
| Insert Size (kb)： | 2.042 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--3.4, 1.5, 0.6, 0.55, 0.5, 0.35; PvuII--6.1, 0.5; HindIII--6.6; BglI--1.8, 1.5, 1.2, 0.6, 0.35, 0.3 (doublet), 0.2. The insert contains the following restriction sites (nt from the 5 end): PstI--519, 528, 864, 1470, 1549; PvuII--859, 1370; StuI--416. Constructed by ligating a 376 bp StuI/ApaI fragment of the genomic clone cbmis15 containing 10 bp of 5 flanking sequence, 121 bp of 5 untranslated region, and 245 bp of the coding region (nt 80-456 of the reported sequence), to a 1666 bp ApaI/HindIII fragment of the cDNA clone pX21 containing the rest of the coding sequence and the 3 untranslated region. pBG311 is an animal cell expression vector constructed from pAT153 and the SV40 early promoter, the t splice signal, and the T polyadenylation signal from pSV2-dhfr. It includes a 44 bp polylinker. |
| References： | Cate RL, et al. Isolation of the bovine and human genes for Mullerian inhibiting substance and expression of the human gene in animal cells. Cell 45: 685-698, 1986. PubMed: 3754790 Cate RL, Donahoe PK. DNA sequences, recombinant DNA molecules and processes for producing mullerian inhibiting substance-like polypeptides. US Patent 5,047,336 dated Sep 10 1991 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pBG311.bmis IVI 10090 |
|---|---|
| 商品货号： | TS149193 |
| Designations： | pBG311.bmis IVI 10090 |
| GenBank Number： | M13151 |
| Species： | Bos taurus, cow |
| Depositors： | Biogen, Inc., RL Cate, Biogen, Inc. |
| Applications： | in another host, produces protein anti-Mullerian hormone (Mullerian inhibiting substance) |
| Vector： | Construct size (kb): 6.535999774932861 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.04200005531311 Tissue: testis Gene product: anti-Mullerian hormone (Mullerian inhibiting substance) AMH Target Gene: anti-Mullerian hormone (Mullerian inhibiting substance) |
| Insert Size (kb)： | 2.042 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--3.4, 1.5, 0.6, 0.55, 0.5, 0.35; PvuII--6.1, 0.5; HindIII--6.6; BglI--1.8, 1.5, 1.2, 0.6, 0.35, 0.3 (doublet), 0.2. The insert contains the following restriction sites (nt from the 5 end): PstI--519, 528, 864, 1470, 1549; PvuII--859, 1370; StuI--416. Constructed by ligating a 376 bp StuI/ApaI fragment of the genomic clone cbmis15 containing 10 bp of 5 flanking sequence, 121 bp of 5 untranslated region, and 245 bp of the coding region (nt 80-456 of the reported sequence), to a 1666 bp ApaI/HindIII fragment of the cDNA clone pX21 containing the rest of the coding sequence and the 3 untranslated region. pBG311 is an animal cell expression vector constructed from pAT153 and the SV40 early promoter, the t splice signal, and the T polyadenylation signal from pSV2-dhfr. It includes a 44 bp polylinker. |
| References： | Cate RL, et al. Isolation of the bovine and human genes for Mullerian inhibiting substance and expression of the human gene in animal cells. Cell 45: 685-698, 1986. PubMed: 3754790 Cate RL, Donahoe PK. DNA sequences, recombinant DNA molecules and processes for producing mullerian inhibiting substance-like polypeptides. US Patent 5,047,336 dated Sep 10 1991 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
