pCN41-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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pCN41
pCN41
规格:
货期:
编号:TS149288
品牌:Testobio
产品名称: pCN41
商品货号: TS149288
Designations: pCN41
Depositors: R Diaz Orejas
Biosafety Level: 1
Vector Information:
Size (kb): 4.5000000000000000
Vector: pCN41 (plasmid)
Construction: pCN40
Marker(s):strR
Construct size (kb): 4.5
Features: marker(s): strR
replicon: repA
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--2.4, 1.1, 1.0; KpnI/SmaI--4.6; PvuII/PstI--2.5, 2.0; KpnI--4.6; SmaI--uncut.
Contains the basic replicon of pPS10 which has been sequenced (EMBL accession number X58896). The repA portion is flanked by EcoRI sites (nt 536 and 1500), with the site at nt 536 between the repA promoter and coding sequences.
General purpose vector.
Constructed by excising a BamHI/HindIII fragment encoding part of kanR from pCN40 (ATCC 77097), blunting with Klenow fragment, and religating.
Media: ATCC Medium 1065 (see below) plus streptomycin ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L
Growth Conditions:
Temperature: 37.0°C
References:

Nieto C, et al. Cloning vectors, derived from a naturally occurring plasmid of Pseudomonas savastanoi, specifically tailored for genetic manipulations in Pseudomonas. Gene 87: 145-149, 1990. PubMed: 2110095

Ramon Diaz Orejas, personal communication

Shipped: freeze-dried
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pCN41

  • 货号: TS149288
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: pCN41
商品货号: TS149288
Designations: pCN41
Depositors: R Diaz Orejas
Biosafety Level: 1
Vector Information:
Size (kb): 4.5000000000000000
Vector: pCN41 (plasmid)
Construction: pCN40
Marker(s):strR
Construct size (kb): 4.5
Features: marker(s): strR
replicon: repA
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--2.4, 1.1, 1.0; KpnI/SmaI--4.6; PvuII/PstI--2.5, 2.0; KpnI--4.6; SmaI--uncut.
Contains the basic replicon of pPS10 which has been sequenced (EMBL accession number X58896). The repA portion is flanked by EcoRI sites (nt 536 and 1500), with the site at nt 536 between the repA promoter and coding sequences.
General purpose vector.
Constructed by excising a BamHI/HindIII fragment encoding part of kanR from pCN40 (ATCC 77097), blunting with Klenow fragment, and religating.
Media: ATCC Medium 1065 (see below) plus streptomycin ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L
Growth Conditions:
Temperature: 37.0°C
References:

Nieto C, et al. Cloning vectors, derived from a naturally occurring plasmid of Pseudomonas savastanoi, specifically tailored for genetic manipulations in Pseudomonas. Gene 87: 145-149, 1990. PubMed: 2110095

Ramon Diaz Orejas, personal communication

Shipped: freeze-dried
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