| 产品名称： | Tetramitus rostratus Bunting |
|---|---|
| 商品货号： | TS151315 |
| Strain Designations： | Tr B |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Derived from same parentage as ATCC 30216. Permanent flagellate. |
| Product Format： | frozen |
| Type Strain： | no |
| Medium： | ATCC® Medium 1251: Tetramitus rostratus flagellate medium, PY/2 |
| Growth Conditions： | Temperature: 25.0°C Duration: grown with Escherichia coli ATCC 11775 |
| Cryopreservation： | 1.xa0 Harvest cells from a culture that is at or near peak density by centrifugation at 800 x g for 5 min. 2.xa0 Adjust the concentration of cells to 2 x 106 - 2 x 107/ml in fresh medium. 3.xa0 While cells are centrifuging prepare a 5% (v/v) solution of sterile DMSO in fresh medium. 4. Mix the cell preparation and the 5% DMSO in equal portions. Thus, the final concentration will be 106 - 107 cells/ml and 2.5% (v/v) DMSO. The time from the mixing of the cell preparation and methanol stock solution to the beginning of the freezing process should be no less than 5 min and no greater than 15 min. 5.xa0 Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation). 6.xa0xa0 Place the vials in a controlled rate freezing unit.xa0 From room temperature cool at -1°C/min to -40°C.xa0 If the freezing unit can compensate for the heat of fusion, maintain rate atxa0xa0xa0xa0xa0xa0xa0 -1°C/min through the heat of fusion.xa0 At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately xa0xa0xa0xa0xa0 -1°C/min.) xa0 7.xa0xa0 Store in either the vapor or liquid phase of a nitrogen refrigerator. 8.xa0xa0 To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes.xa0 Do not agitate the ampule.xa0 Do not leave ampule in water bath after thawed. 9.xa0xa0 Immediately after thawing, aseptically transfer contents to a 16 x 125 mm screw-capped test tube containing 7 ml of ATCC Medium 1251. 10.xa0xa0xa0xa0xa0xa0xa0xa0xa0 Incubate the tube upright with the cap screwed on loosely (loosened one half turn) at 25°C. |
| Name of Depositor： | W Balamuth |
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| 产品名称： | Tetramitus rostratus Bunting |
|---|---|
| 商品货号： | TS151315 |
| Strain Designations： | Tr B |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Derived from same parentage as ATCC 30216. Permanent flagellate. |
| Product Format： | frozen |
| Type Strain： | no |
| Medium： | ATCC® Medium 1251: Tetramitus rostratus flagellate medium, PY/2 |
| Growth Conditions： | Temperature: 25.0°C Duration: grown with Escherichia coli ATCC 11775 |
| Cryopreservation： | 1.xa0 Harvest cells from a culture that is at or near peak density by centrifugation at 800 x g for 5 min. 2.xa0 Adjust the concentration of cells to 2 x 106 - 2 x 107/ml in fresh medium. 3.xa0 While cells are centrifuging prepare a 5% (v/v) solution of sterile DMSO in fresh medium. 4. Mix the cell preparation and the 5% DMSO in equal portions. Thus, the final concentration will be 106 - 107 cells/ml and 2.5% (v/v) DMSO. The time from the mixing of the cell preparation and methanol stock solution to the beginning of the freezing process should be no less than 5 min and no greater than 15 min. 5.xa0 Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation). 6.xa0xa0 Place the vials in a controlled rate freezing unit.xa0 From room temperature cool at -1°C/min to -40°C.xa0 If the freezing unit can compensate for the heat of fusion, maintain rate atxa0xa0xa0xa0xa0xa0xa0 -1°C/min through the heat of fusion.xa0 At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately xa0xa0xa0xa0xa0 -1°C/min.) xa0 7.xa0xa0 Store in either the vapor or liquid phase of a nitrogen refrigerator. 8.xa0xa0 To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes.xa0 Do not agitate the ampule.xa0 Do not leave ampule in water bath after thawed. 9.xa0xa0 Immediately after thawing, aseptically transfer contents to a 16 x 125 mm screw-capped test tube containing 7 ml of ATCC Medium 1251. 10.xa0xa0xa0xa0xa0xa0xa0xa0xa0 Incubate the tube upright with the cap screwed on loosely (loosened one half turn) at 25°C. |
| Name of Depositor： | W Balamuth |
