J-Bir [pERT87-J-Bir]-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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J-Bir [pERT87-J-Bir]
J-Bir [pERT87-J-Bir]
规格:
货期:
编号:TS151508
品牌:Testobio
产品名称: J-Bir pERT87-J-Bir
商品货号: TS151508
Designations: J-Bir pERT87-J-Bir
Species: Homo sapiens, human
Depositors: LM Kunkel
Vector:
Construct size (kb): 6.21999979019165
Insert:
DNA: genomic
Insert lengths(kb): 3.0
Gene product: DNA Segment, single copy (within DMD gene) DXS270
Alleles: A2, A1
Insert Size (kb): 3.0
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): HindIII/KpnI--3.3, 1.94, 1.25; HindIII--3.45, 3.2; BamHI--4.0, 2.8; KpnI--4.36, 2.03; consistent with deposited DNA.
The fragment is within the DMD locus.
A HindIII/KpnI digestion yields 2 fragments--one at 1.8-1.9 and the other at 1.15. The latter detects the RFLP, the former is slightly repeated.
The polymorphisms detected by this probe are contained within the DMD locus.
Care should be taken to assure efficient transfer of the larger allele.
References:

Koenig M, et al. Complete cloning of the Duchenne muscular dystrophy (DMD) cDNA and preliminary genomic organization of the DMD gene in normal and affected individuals. Cell 50: 509-517, 1987. PubMed: 3607877

Monaco AP, et al. Localization and cloning of Xp21 deletion breakpoints involved in muscular dystrophy. Hum. Genet. 75: 221-227, 1987. PubMed: 2881877

Louis M Kunkel, personal communication

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J-Bir [pERT87-J-Bir]

  • 货号: TS151508
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: J-Bir pERT87-J-Bir
商品货号: TS151508
Designations: J-Bir pERT87-J-Bir
Species: Homo sapiens, human
Depositors: LM Kunkel
Vector:
Construct size (kb): 6.21999979019165
Insert:
DNA: genomic
Insert lengths(kb): 3.0
Gene product: DNA Segment, single copy (within DMD gene) DXS270
Alleles: A2, A1
Insert Size (kb): 3.0
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): HindIII/KpnI--3.3, 1.94, 1.25; HindIII--3.45, 3.2; BamHI--4.0, 2.8; KpnI--4.36, 2.03; consistent with deposited DNA.
The fragment is within the DMD locus.
A HindIII/KpnI digestion yields 2 fragments--one at 1.8-1.9 and the other at 1.15. The latter detects the RFLP, the former is slightly repeated.
The polymorphisms detected by this probe are contained within the DMD locus.
Care should be taken to assure efficient transfer of the larger allele.
References:

Koenig M, et al. Complete cloning of the Duchenne muscular dystrophy (DMD) cDNA and preliminary genomic organization of the DMD gene in normal and affected individuals. Cell 50: 509-517, 1987. PubMed: 3607877

Monaco AP, et al. Localization and cloning of Xp21 deletion breakpoints involved in muscular dystrophy. Hum. Genet. 75: 221-227, 1987. PubMed: 2881877

Louis M Kunkel, personal communication

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