L10BIOBR-GFP-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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L10BIOBR-GFP
L10BIOBR-GFP
规格:
货期:
编号:TS151887
品牌:Testobio
产品名称： L10BIOBR-GFP
商品货号： TS151887
Organism： Mus musculus, mouse
Cell Type： melanocyte
Product Format： frozen
Morphology： melanocyte
Culture Properties： adherent
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age： newborn
Strain： B10.BR
Applications：
Together, L10BIOBR-GFP (CRL-2770) and L10BIOBR-MAPKK (ATCC® CRL-2771) are valuable cell models for oncogenic transformation and signal transduction studies for melanoma PubMed: 12514183.
The L10BIOBR-GFP cell line was derived as a negative control for CRL-2771. The immortalized murine melanocyte cell line L10BIOBR was transduced with pDIVA-GFP and subjected to puromycin selection.
Please note, although the L10BIOBR-GFP cell line harbors the gfp gene, as verified by PCR analysis, the cell line does not express sufficient GFP protein for detection of GFP fluorescence by flow cytometry or fluorescence microscopy.
Storage Conditions： liquid nitrogen vapor phase
Images：
Derivation：
The L10BIOBR-GFP cell line was derived as a negative control for CRL-2771. The immortalized murine melanocyte cell line L10BIOBR was transduced with pDIVA-GFP and subjected to puromycin selection. Together, L10BIOBR-GFP (CRL-2770) and L10BIOBR-MAPKK (ATCC® CRL-2771) are valuable cell models for oncogenic transformation and signal transduction studies for melanoma PubMed: 12514183. Please note, although the L10BIOBR-GFP cell line harbors the gfp gene, as verified by PCR analysis, the cell line does not express sufficient GFP protein for detection of GFP fluorescence by flow cytometry or fluorescence microscopy.
Tumorigenic： No
Effects：
No, progressive tumor growth was not observed in nude mice
RefGovindarajan B, et al. Malignant transformation of melanocytes to melanoma by constitutive activation of mitogen-activated protein kinase kinase (MAPKK) signaling. J. Biol. Chem. 278: 9790-9795, 2003. PubMed: 12514183
Comments：
The L10BIOBR-GFP cell line was derived as a negative control for CRL-2771. The immortalized murine melanocyte cell line L10BIOBR was transduced with pDIVA-GFP and subjected to puromycin selection. Together, L10BIOBR-GFP (CRL-2770) and L10BIOBR-MAPKK (ATCC® CRL-2771) are valuable cell models for oncogenic transformation and signal transduction studies for melanoma PubMed: 12514183. Please note, although the L10BIOBR-GFP cell line harbors the gfp gene, as verified by PCR analysis, the cell line does not express sufficient GFP protein for detection of GFP fluorescence by flow cytometry or fluorescence microscopy.
Complete Growth Medium： Hams F10 medium supplemented with 50 ng/ml TPA (Sigma Catalogue No. P-8139) and 7% horse serum
Subculturing：
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
    An inoculum of 5 X 10(3) to 7 X 10(3) viable cells/sq. cm. is recommended.
  6. Incubate cultures at 37°C.
Interval: Subculture when cells reach a concentration of 2 X 10(4) cells/sq. cm.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Two to three times weekly
Cryopreservation：
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions：
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Population Doubling Time： 29 hours
Name of Depositor： JL Arbiser
Year of Origin： January 1, 2002
References：

Govindarajan B, et al. Malignant transformation of melanocytes to melanoma by constitutive activation of mitogen-activated protein kinase kinase (MAPKK) signaling. J. Biol. Chem. 278: 9790-9795, 2003. PubMed: 12514183

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L10BIOBR-GFP

  • 货号: TS151887
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称： L10BIOBR-GFP
商品货号： TS151887
Organism： Mus musculus, mouse
Cell Type： melanocyte
Product Format： frozen
Morphology： melanocyte
Culture Properties： adherent
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age： newborn
Strain： B10.BR
Applications：
Together, L10BIOBR-GFP (CRL-2770) and L10BIOBR-MAPKK (ATCC® CRL-2771) are valuable cell models for oncogenic transformation and signal transduction studies for melanoma PubMed: 12514183.
The L10BIOBR-GFP cell line was derived as a negative control for CRL-2771. The immortalized murine melanocyte cell line L10BIOBR was transduced with pDIVA-GFP and subjected to puromycin selection.
Please note, although the L10BIOBR-GFP cell line harbors the gfp gene, as verified by PCR analysis, the cell line does not express sufficient GFP protein for detection of GFP fluorescence by flow cytometry or fluorescence microscopy.
Storage Conditions： liquid nitrogen vapor phase
Images：
Derivation：
The L10BIOBR-GFP cell line was derived as a negative control for CRL-2771. The immortalized murine melanocyte cell line L10BIOBR was transduced with pDIVA-GFP and subjected to puromycin selection. Together, L10BIOBR-GFP (CRL-2770) and L10BIOBR-MAPKK (ATCC® CRL-2771) are valuable cell models for oncogenic transformation and signal transduction studies for melanoma PubMed: 12514183. Please note, although the L10BIOBR-GFP cell line harbors the gfp gene, as verified by PCR analysis, the cell line does not express sufficient GFP protein for detection of GFP fluorescence by flow cytometry or fluorescence microscopy.
Tumorigenic： No
Effects：
No, progressive tumor growth was not observed in nude mice
RefGovindarajan B, et al. Malignant transformation of melanocytes to melanoma by constitutive activation of mitogen-activated protein kinase kinase (MAPKK) signaling. J. Biol. Chem. 278: 9790-9795, 2003. PubMed: 12514183
Comments：
The L10BIOBR-GFP cell line was derived as a negative control for CRL-2771. The immortalized murine melanocyte cell line L10BIOBR was transduced with pDIVA-GFP and subjected to puromycin selection. Together, L10BIOBR-GFP (CRL-2770) and L10BIOBR-MAPKK (ATCC® CRL-2771) are valuable cell models for oncogenic transformation and signal transduction studies for melanoma PubMed: 12514183. Please note, although the L10BIOBR-GFP cell line harbors the gfp gene, as verified by PCR analysis, the cell line does not express sufficient GFP protein for detection of GFP fluorescence by flow cytometry or fluorescence microscopy.
Complete Growth Medium： Hams F10 medium supplemented with 50 ng/ml TPA (Sigma Catalogue No. P-8139) and 7% horse serum
Subculturing：
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
    An inoculum of 5 X 10(3) to 7 X 10(3) viable cells/sq. cm. is recommended.
  6. Incubate cultures at 37°C.
Interval: Subculture when cells reach a concentration of 2 X 10(4) cells/sq. cm.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Two to three times weekly
Cryopreservation：
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions：
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Population Doubling Time： 29 hours
Name of Depositor： JL Arbiser
Year of Origin： January 1, 2002
References：

Govindarajan B, et al. Malignant transformation of melanocytes to melanoma by constitutive activation of mitogen-activated protein kinase kinase (MAPKK) signaling. J. Biol. Chem. 278: 9790-9795, 2003. PubMed: 12514183

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李晶晶：13375187189
周雪：13372109301
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