| 产品名称： | pHPGK-7e |
|---|---|
| 商品货号： | TS152180 |
| Designations： | pHPGK-7e |
| GenBank Number： | J00288 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 5.599999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.799999952316284 Tissue: fetal liver Gene product: phosphoglycerate kinase 2( phosphoglycerate kinase 1, pseudogene 2, 2-phosphoglycerate kinase) PGK1 Alleles: B17, B21, A1, A1, A2, , A2 |
| Insert Size (kb)： | 1.800 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--3.7, 1.8; EcoRI--5.6; HindIII--3.9, 1.0, 0.7; BamHI--5.6. Linked to X-linked hypohidrotic ectodermal dysplasia (theta = 0.02, z = 13.44). No mouse Pgk-1 pseudogene is on the X chromosome, and the mouse genome contains several more pseudogenes than does the human genome. Includes 82 bp of 5 and 437 bp of 3 untranslated sequence, the complete coding region, and a polyadenylation signal 17 bp from the poly(A) site. The PstI RFLP is impossible to detect because of an overlapping 6.0 kb autosomal fragment. The autosomal fragment has an XbaI site so genomic DNA can be digested with XbaI/PstI. This RFLP showed X-linked inheritance in 10 families. Enzyme(s) not detecting polymorphism: EcoRI, HindII, MspI, TaqI, AvaII, HindIII, SacI, BamHI, MboII, HpaI. |
| References： | Kazazian HH Jr., et al. Restriction site polymorphism in the phosphoglycerate kinase gene on the X chromosome. Hum. Genet. 66: 217-219, 1984. PubMed: 6325324 Adra CN, et al. The family of mouse phosphoglycerate kinase genes and pseudogenes. Somatic Cell Mol. Genet. 14: 69-81, 1988. PubMed: 2829366 Michelson AM, et al. Isolation and DNA sequence of a full-length cDNA clone for human X chromosome-encoded phosphoglycerate kinase. Proc. Natl. Acad. Sci. USA 80: 472-476, 1983. PubMed: 6188151 Zonana J, et al. X-linked hypohidrotic ectodermal dysplasia: localization within the region Xq11-21.1 by linkage analysis and implications for carrier detection and prenatal diagnosis. Am. J. Hum. Genet. 43: 75-85, 1988. PubMed: 3163892 |
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| 产品名称： | pHPGK-7e |
|---|---|
| 商品货号： | TS152180 |
| Designations： | pHPGK-7e |
| GenBank Number： | J00288 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 5.599999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.799999952316284 Tissue: fetal liver Gene product: phosphoglycerate kinase 2( phosphoglycerate kinase 1, pseudogene 2, 2-phosphoglycerate kinase) PGK1 Alleles: B17, B21, A1, A1, A2, , A2 |
| Insert Size (kb)： | 1.800 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--3.7, 1.8; EcoRI--5.6; HindIII--3.9, 1.0, 0.7; BamHI--5.6. Linked to X-linked hypohidrotic ectodermal dysplasia (theta = 0.02, z = 13.44). No mouse Pgk-1 pseudogene is on the X chromosome, and the mouse genome contains several more pseudogenes than does the human genome. Includes 82 bp of 5 and 437 bp of 3 untranslated sequence, the complete coding region, and a polyadenylation signal 17 bp from the poly(A) site. The PstI RFLP is impossible to detect because of an overlapping 6.0 kb autosomal fragment. The autosomal fragment has an XbaI site so genomic DNA can be digested with XbaI/PstI. This RFLP showed X-linked inheritance in 10 families. Enzyme(s) not detecting polymorphism: EcoRI, HindII, MspI, TaqI, AvaII, HindIII, SacI, BamHI, MboII, HpaI. |
| References： | Kazazian HH Jr., et al. Restriction site polymorphism in the phosphoglycerate kinase gene on the X chromosome. Hum. Genet. 66: 217-219, 1984. PubMed: 6325324 Adra CN, et al. The family of mouse phosphoglycerate kinase genes and pseudogenes. Somatic Cell Mol. Genet. 14: 69-81, 1988. PubMed: 2829366 Michelson AM, et al. Isolation and DNA sequence of a full-length cDNA clone for human X chromosome-encoded phosphoglycerate kinase. Proc. Natl. Acad. Sci. USA 80: 472-476, 1983. PubMed: 6188151 Zonana J, et al. X-linked hypohidrotic ectodermal dysplasia: localization within the region Xq11-21.1 by linkage analysis and implications for carrier detection and prenatal diagnosis. Am. J. Hum. Genet. 43: 75-85, 1988. PubMed: 3163892 |
