pSE2.0-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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pSE2.0
pSE2.0
规格:
货期:
编号:TS152441
品牌:Testobio
产品名称: pSE2.0
商品货号: TS152441
Designations: pSE2.0
Species: Homo sapiens, human
Applications:
Has been used successfully to detect transcripts in Northern blots.
Vector:
Construct size (kb): 5.0
Insert:
DNA: genomic
Insert lengths(kb): 2.0
Gene product: ribonucleotide reductase M1 polypeptide(ribonucleoside diphosphate reductase, M1 subunit) RRM1
Alleles: A2, A2, A1
Insert Size (kb): 2.0
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
The insert does not contain EcoRI, KpnI, SacI or BamHI sites. The SacI and XhoI sites from the multiple cloning site of the original lambda vector GEM-11 were not removed and are there.
The insert contains a HindIII site 1.3 kb from the EcoRI end and another HindIII site 0.1 kb from the SacI end.
Restriction digests of the clone give the following sizes (kb): EcoRI/SacI--3.0, 2.0; HindIII--4.4, 0.6; PstI--uncut; BamHI--5.0; XhoI--3.0, 2.0.
Detects a genomic SacI fragment of 18 kb.
Requires preassociation with sheared human DNA prior to hybridization for optimal results. Has been used successfully to detect transcripts in Northern blots.
References:

Jennifer A Byrne, personal communication

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pSE2.0

  • 货号: TS152441
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: pSE2.0
商品货号: TS152441
Designations: pSE2.0
Species: Homo sapiens, human
Applications:
Has been used successfully to detect transcripts in Northern blots.
Vector:
Construct size (kb): 5.0
Insert:
DNA: genomic
Insert lengths(kb): 2.0
Gene product: ribonucleotide reductase M1 polypeptide(ribonucleoside diphosphate reductase, M1 subunit) RRM1
Alleles: A2, A2, A1
Insert Size (kb): 2.0
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
The insert does not contain EcoRI, KpnI, SacI or BamHI sites. The SacI and XhoI sites from the multiple cloning site of the original lambda vector GEM-11 were not removed and are there.
The insert contains a HindIII site 1.3 kb from the EcoRI end and another HindIII site 0.1 kb from the SacI end.
Restriction digests of the clone give the following sizes (kb): EcoRI/SacI--3.0, 2.0; HindIII--4.4, 0.6; PstI--uncut; BamHI--5.0; XhoI--3.0, 2.0.
Detects a genomic SacI fragment of 18 kb.
Requires preassociation with sheared human DNA prior to hybridization for optimal results. Has been used successfully to detect transcripts in Northern blots.
References:

Jennifer A Byrne, personal communication

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