| 产品名称： | CHLA-04-ATRT |
|---|---|
| 商品货号： | TS152678 |
| Organism： | Homo sapiens, human |
| Tissue： | Brain (lateral and third ventricle) |
| Product Format： | frozen |
| Morphology： | rounded |
| Culture Properties： | single cells and clusters in suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Atypical Teratoid Rhabdoid Tumor (ATRT) |
| Age： | 20 months |
| Gender： | Male |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | This is a human diploid cell line of male origin in which most of the examined cells contained the karyotypically normal male chromosome complement, 46,XY. |
| Images： |  |
| Derivation： | CHLA-04-ATRT was established from a 20 month old boy with recurrent atypical teratoid rhabdoid tumor (ATRT) with wide dissemination in the brain and CSF.xa0 Cells were isolated from the brain tumor at the time of autopsy and were cultured in neurobasal medium after mechanical disruption. |
| Clinical Data： | 20 months old male |
| Antigen Expression： | epithelial membrane antigen (EMA1), expressed |
| Comments： | This cell line has been continuously carried in culture for over 8 months, and in neurobasal medium grows as spheres of varying sizes. ATCC has verified that CHLA-04-ATRT expresses epithelial membrane antigen (EMA1) and does not express INI-1. The generation of this cell line was made possible with the support of the Michael Hoefflin Foundation for Childrens Cancer to Children’s Hospital Los Angeles, with the goal of making pediatric brain tumor lines available to the research community. |
| Complete Growth Medium： | DMEM:F12 Medium (ATCC 30-2006) with 20 ng/mL human recombinant EGF, 20 ng/mL human recombinant basic FGF, and B-27 Supplement (Invitrogen, Cat. No.17504) to a final concentration of 2% (v/v)
|
| Subculturing： | Cultures can be maintained by the addition of fresh medium when there are numerous, healthy-appearing clusters present in suspension and pH of medium has decreased. Alternatively, cultures can be established by centrifugation with subsequent resuspension in ¼ volume of the conditioned medium and ¾ volume fresh medium. |
| Cryopreservation： | Freeze medium: Fetal bovine serum, 90%; DMSO, 10% (v/v).
Cell culture tested DMSO is available as ATCC 4-X
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | D5S818: 12 D13S317: 12 D7S820: 11 D16S539: 11 vWA: 17 TH01: 6, 9 Amelogenin: X Y TPOX: 8 CSF1PO: 11, 13 |
| Name of Depositor： | A Erdreich-Epstein, Saban Research Institute, Children’s Hospital Los Angeles |
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| 产品名称： | CHLA-04-ATRT |
|---|---|
| 商品货号： | TS152678 |
| Organism： | Homo sapiens, human |
| Tissue： | Brain (lateral and third ventricle) |
| Product Format： | frozen |
| Morphology： | rounded |
| Culture Properties： | single cells and clusters in suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Atypical Teratoid Rhabdoid Tumor (ATRT) |
| Age： | 20 months |
| Gender： | Male |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | This is a human diploid cell line of male origin in which most of the examined cells contained the karyotypically normal male chromosome complement, 46,XY. |
| Images： | |
| Derivation： | CHLA-04-ATRT was established from a 20 month old boy with recurrent atypical teratoid rhabdoid tumor (ATRT) with wide dissemination in the brain and CSF.xa0 Cells were isolated from the brain tumor at the time of autopsy and were cultured in neurobasal medium after mechanical disruption. |
| Clinical Data： | 20 months old male |
| Antigen Expression： | epithelial membrane antigen (EMA1), expressed |
| Comments： | This cell line has been continuously carried in culture for over 8 months, and in neurobasal medium grows as spheres of varying sizes. ATCC has verified that CHLA-04-ATRT expresses epithelial membrane antigen (EMA1) and does not express INI-1. The generation of this cell line was made possible with the support of the Michael Hoefflin Foundation for Childrens Cancer to Children’s Hospital Los Angeles, with the goal of making pediatric brain tumor lines available to the research community. |
| Complete Growth Medium： | DMEM:F12 Medium (ATCC 30-2006) with 20 ng/mL human recombinant EGF, 20 ng/mL human recombinant basic FGF, and B-27 Supplement (Invitrogen, Cat. No.17504) to a final concentration of 2% (v/v)
|
| Subculturing： | Cultures can be maintained by the addition of fresh medium when there are numerous, healthy-appearing clusters present in suspension and pH of medium has decreased. Alternatively, cultures can be established by centrifugation with subsequent resuspension in ¼ volume of the conditioned medium and ¾ volume fresh medium. |
| Cryopreservation： | Freeze medium: Fetal bovine serum, 90%; DMSO, 10% (v/v).
Cell culture tested DMSO is available as ATCC 4-X
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | D5S818: 12 D13S317: 12 D7S820: 11 D16S539: 11 vWA: 17 TH01: 6, 9 Amelogenin: X Y TPOX: 8 CSF1PO: 11, 13 |
| Name of Depositor： | A Erdreich-Epstein, Saban Research Institute, Children’s Hospital Los Angeles |
