pKSM711
pKSM711
规格:
货期:
编号:TS153279
品牌:Testobio
| 产品名称： | pKSM711 |
|---|---|
| 商品货号： | TS153279 |
| Designations： | pKSM711 |
| Depositors： | K Ippen-Ihler, S Maneewannakul |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.7409999370574950 Vector: pKSM711 (phagemid) Promoters: Promoter for in vitro transcription T7lac Construction: pKSM710, pUC4-KIXX Marker(s):kanR Construct size (kb): 3.740999937057495 Features: insert detection: lacZalpha marker(s): kanR promoter for expression: lacUV5 promoter for in vitro transcription: T7lac replicon: fd replicon: pMB1 MCS: BamHI...EcoRI transcription terminator: T7 transcription terminator: fd transcription terminator: rrnB |
| Applications： | expression vector vector permitting RNA synthesis in vitro vector permitting production of single-stranded DNA vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.7; EcoRI--3.7; PstI--3.7. Vector contains the following restriction sites (approximate kb from nt 1): BamHI--0.34; BglII--3.42; EcoRI--0.39; NcoI--0.33, 2.82; PstI--3.20; PvuI--0.51; PvuII--0.48, 3.15, 3.51. Differs from pKSM712 (ATCC 87158) only in the orientation of the kanamycin resistance gene. Expression vector. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Maneewannakul S, et al. The pKSM710 vector cassette provides tightly regulated lac and T7lac promoters and strategies for manipulating N-terminal protein sequences. Plasmid 31: 300-307, 1994. PubMed: 8058823 |
| Shipped： | freeze-dried |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
pKSM711
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | pKSM711 |
|---|---|
| 商品货号： | TS153279 |
| Designations： | pKSM711 |
| Depositors： | K Ippen-Ihler, S Maneewannakul |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.7409999370574950 Vector: pKSM711 (phagemid) Promoters: Promoter for in vitro transcription T7lac Construction: pKSM710, pUC4-KIXX Marker(s):kanR Construct size (kb): 3.740999937057495 Features: insert detection: lacZalpha marker(s): kanR promoter for expression: lacUV5 promoter for in vitro transcription: T7lac replicon: fd replicon: pMB1 MCS: BamHI...EcoRI transcription terminator: T7 transcription terminator: fd transcription terminator: rrnB |
| Applications： | expression vector vector permitting RNA synthesis in vitro vector permitting production of single-stranded DNA vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.7; EcoRI--3.7; PstI--3.7. Vector contains the following restriction sites (approximate kb from nt 1): BamHI--0.34; BglII--3.42; EcoRI--0.39; NcoI--0.33, 2.82; PstI--3.20; PvuI--0.51; PvuII--0.48, 3.15, 3.51. Differs from pKSM712 (ATCC 87158) only in the orientation of the kanamycin resistance gene. Expression vector. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Maneewannakul S, et al. The pKSM710 vector cassette provides tightly regulated lac and T7lac promoters and strategies for manipulating N-terminal protein sequences. Plasmid 31: 300-307, 1994. PubMed: 8058823 |
| Shipped： | freeze-dried |
