| 产品名称： | pACYC177 |
|---|---|
| 商品货号： | TS153556 |
| Designations： | pACYC177 |
| Depositors： | SN Cohen |
| Other IDs： | Nucleotide (GenBank) : VB0109 Cloning vector pACYC177. |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.9400000572204590 Vector: pACYC177 (plasmid) Construction: p15A, Tn903, Tn3 Marker(s):ampR,kanR Construct size (kb): 3.940000057220459 Features: marker(s): ampR, kanR replicon: p15A |
| Comments： | Low copy number plasmid vector containing the replication system of miniplasmid p15A. When HincII is nucleotide 1, Tn3 region extends from 3026-756 and beta-lactamase from 369-615. The promoter and part of the coding region for the Tn3 repressor (3025-3515) have been deleted. The Tn903 (1595-3025) region includes kanR (1922-2734). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Chang AC, Cohen SN. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J. Bacteriol. 134: 1141-1156, 1978. PubMed: 149110 Rose RE. The nucleotide sequence of pACYC177. Nucleic Acids Res. 16: 356, 1988. PubMed: 3340534 |
| Shipped： | frozen |
| Shipping Information： | Shipped: xa0Frozen glycerol stock of xa0E. col containing the plasmid. |
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| 产品名称： | pACYC177 |
|---|---|
| 商品货号： | TS153556 |
| Designations： | pACYC177 |
| Depositors： | SN Cohen |
| Other IDs： | Nucleotide (GenBank) : VB0109 Cloning vector pACYC177. |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.9400000572204590 Vector: pACYC177 (plasmid) Construction: p15A, Tn903, Tn3 Marker(s):ampR,kanR Construct size (kb): 3.940000057220459 Features: marker(s): ampR, kanR replicon: p15A |
| Comments： | Low copy number plasmid vector containing the replication system of miniplasmid p15A. When HincII is nucleotide 1, Tn3 region extends from 3026-756 and beta-lactamase from 369-615. The promoter and part of the coding region for the Tn3 repressor (3025-3515) have been deleted. The Tn903 (1595-3025) region includes kanR (1922-2734). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Chang AC, Cohen SN. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J. Bacteriol. 134: 1141-1156, 1978. PubMed: 149110 Rose RE. The nucleotide sequence of pACYC177. Nucleic Acids Res. 16: 356, 1988. PubMed: 3340534 |
| Shipped： | frozen |
| Shipping Information： | Shipped: xa0Frozen glycerol stock of xa0E. col containing the plasmid. |
