Sp3-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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Sp3
Sp3
规格:
货期:
编号:TS153877
品牌:Testobio
产品名称: Sp3
商品货号: TS153877
Designations: Sp3
GenBank Number:

M97191

Species: Homo sapiens, human
Applications:
contains sequence Sp3 transcription factor
Vector:
Construct size (kb): 4.900000095367432
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSP72
Intact vector size: 2.462
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: XhoI PvuII HindIII SphI PstI SalI AccI XbaI BamHI SmaI KpnI
SacI EcoRI ClaI EcoRV BglII
Polylinker sites: XhoI PvuII HindIII SphI PstI SalI AccI XbaI BamHI SmaI KpnI
SacI EcoRI ClaI EcoRV BglII
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
promoter: SP6, T7
replicon: pMB1
Cross references: DNA Seq. Acc.: X65332
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SP3
Gene name: Sp3 transcription factor
Contains complete coding sequence?: Y
Tissue: Hut78 cell line, PMA activated
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 2.4
Cross references: DNA Seq. Acc.: M97191
Nucleotides 1-2221 of the insert correspond to
nucleotides 1-2221 of M97191.
Insert lengths(kb): 2.400000095367432
Tissue: Hut78 cell line, PMA activated
Gene product: Sp3 transcription factor SP3
Target Gene: Sp3 transcription factor
Insert Size (kb): 2.400
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): BglII--3.8, 0.96; EcoRI--2.5, 2.2; NotI--4.7; PstI--3.0, 0.8, 0.6, 0.3; XbaI--4.7.
Insert contains the following restriction sites (approximate kb from the 5 end): BglII--0.89; EcoRI--0.0, 2.22; EcoRV--2.07; KpnI--1.63; NcoI--1.06; NotI--0.04; PstI--0.66, 1.43, 1.98; PvuII--1.12; SacI--1.71; XbaI--0.62.
References:

Kingsley C, Winoto A. Cloning of GT box-binding proteins: a novel Sp1 multigene family regulating T-cell receptor gene expression. Mol. Cell. Biol. 12: 4251-4261, 1992. PubMed: 1341900

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Sp3

  • 货号: TS153877
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: Sp3
商品货号: TS153877
Designations: Sp3
GenBank Number:

M97191

Species: Homo sapiens, human
Applications:
contains sequence Sp3 transcription factor
Vector:
Construct size (kb): 4.900000095367432
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSP72
Intact vector size: 2.462
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: XhoI PvuII HindIII SphI PstI SalI AccI XbaI BamHI SmaI KpnI
SacI EcoRI ClaI EcoRV BglII
Polylinker sites: XhoI PvuII HindIII SphI PstI SalI AccI XbaI BamHI SmaI KpnI
SacI EcoRI ClaI EcoRV BglII
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
promoter: SP6, T7
replicon: pMB1
Cross references: DNA Seq. Acc.: X65332
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SP3
Gene name: Sp3 transcription factor
Contains complete coding sequence?: Y
Tissue: Hut78 cell line, PMA activated
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 2.4
Cross references: DNA Seq. Acc.: M97191
Nucleotides 1-2221 of the insert correspond to
nucleotides 1-2221 of M97191.
Insert lengths(kb): 2.400000095367432
Tissue: Hut78 cell line, PMA activated
Gene product: Sp3 transcription factor SP3
Target Gene: Sp3 transcription factor
Insert Size (kb): 2.400
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): BglII--3.8, 0.96; EcoRI--2.5, 2.2; NotI--4.7; PstI--3.0, 0.8, 0.6, 0.3; XbaI--4.7.
Insert contains the following restriction sites (approximate kb from the 5 end): BglII--0.89; EcoRI--0.0, 2.22; EcoRV--2.07; KpnI--1.63; NcoI--1.06; NotI--0.04; PstI--0.66, 1.43, 1.98; PvuII--1.12; SacI--1.71; XbaI--0.62.
References:

Kingsley C, Winoto A. Cloning of GT box-binding proteins: a novel Sp1 multigene family regulating T-cell receptor gene expression. Mol. Cell. Biol. 12: 4251-4261, 1992. PubMed: 1341900

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