| 产品名称： | pCR1-1 CR1-1 |
|---|---|
| 商品货号： | TS154011 |
| Designations： | pCR1-1 CR1-1 |
| GenBank Number： | M11569 |
| Species： | Homo sapiens, human |
| Depositors： | WW Wong |
| Applications： | Can be used to detect CR2-specific sequences at low stringency. |
| Vector： | Construct size (kb): 4.099999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.7900000214576721 Tissue: tonsil Gene product: complement component (3b/4b) receptor 1 CR1 Alleles: H2, D1, D2, H1, I1, I2 |
| Insert Size (kb)： | 0.790 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Sequence of this probe is given in Fig. 2, nucleotides 523-1314; encodes portion of amino acid sequence termed long homologous repeat B (LHR-B); contains coding sequences for CR1 tryptic peptides 41c and 34b. The insert detects mRNAs of 9 kb and 11 kb. It also detects multiple bands in BamHI and HindIII digests. HindIII RFLP correlates with CR1 expression on erythrocytes (7.4 high, 6.9 low). Can be used to detect CR2-specific sequences at low stringency. Enzyme(s) not detecting polymorphism (in a panel of 5): BamHI, BglII, HIncII, HindIII, PstI, RsaI, TaqI. |
| References： | Weis JH, et al. A complement receptor locus: genes encoding C3b/C4b receptor and C3d/Epstein-Barr virus receptor map to 1q32. J. Immunol. 138: 312-315, 1987. PubMed: 3782802 Wong WW, et al. Identification of a partial cDNA clone for the human receptor for complement fragments C3b/C4b. Proc. Natl. Acad. Sci. USA 82: 7711-7715, 1985. PubMed: 2933745 Wong WW, et al. Analysis of multiple restriction fragment length polymorphisms of the gene for the human complement receptor type I. Duplication of genomic sequences occurs in association with a high molecular mass receptor allotype. J. Exp. Med. 164: 1531-1546, 1986. PubMed: 2877046 Wilson JG, et al. Identification of a restriction fragment length polymorphism by a CR1 cDNA that correlates with the number of CR1 on erythrocytes. J. Exp. Med. 164: 50-59, 1986. PubMed: 3014040 Buetow KH, et al. A detailed multipoint gene map of chromosome 1q. Genomics 8: 13-21, 1990. PubMed: 1981991 Klickstein LB, et al. Human C3b/C4b receptor (CR1). Demonstration of long homologous repeating domains that are composed of the short consensus repeats characteristic of C3/C4 binding proteins. J. Exp. Med. 165: 1095-1112, 1987. PubMed: 2951479 Barbara Weiffenbach, personal communication |
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| 产品名称： | pCR1-1 CR1-1 |
|---|---|
| 商品货号： | TS154011 |
| Designations： | pCR1-1 CR1-1 |
| GenBank Number： | M11569 |
| Species： | Homo sapiens, human |
| Depositors： | WW Wong |
| Applications： | Can be used to detect CR2-specific sequences at low stringency. |
| Vector： | Construct size (kb): 4.099999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.7900000214576721 Tissue: tonsil Gene product: complement component (3b/4b) receptor 1 CR1 Alleles: H2, D1, D2, H1, I1, I2 |
| Insert Size (kb)： | 0.790 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Sequence of this probe is given in Fig. 2, nucleotides 523-1314; encodes portion of amino acid sequence termed long homologous repeat B (LHR-B); contains coding sequences for CR1 tryptic peptides 41c and 34b. The insert detects mRNAs of 9 kb and 11 kb. It also detects multiple bands in BamHI and HindIII digests. HindIII RFLP correlates with CR1 expression on erythrocytes (7.4 high, 6.9 low). Can be used to detect CR2-specific sequences at low stringency. Enzyme(s) not detecting polymorphism (in a panel of 5): BamHI, BglII, HIncII, HindIII, PstI, RsaI, TaqI. |
| References： | Weis JH, et al. A complement receptor locus: genes encoding C3b/C4b receptor and C3d/Epstein-Barr virus receptor map to 1q32. J. Immunol. 138: 312-315, 1987. PubMed: 3782802 Wong WW, et al. Identification of a partial cDNA clone for the human receptor for complement fragments C3b/C4b. Proc. Natl. Acad. Sci. USA 82: 7711-7715, 1985. PubMed: 2933745 Wong WW, et al. Analysis of multiple restriction fragment length polymorphisms of the gene for the human complement receptor type I. Duplication of genomic sequences occurs in association with a high molecular mass receptor allotype. J. Exp. Med. 164: 1531-1546, 1986. PubMed: 2877046 Wilson JG, et al. Identification of a restriction fragment length polymorphism by a CR1 cDNA that correlates with the number of CR1 on erythrocytes. J. Exp. Med. 164: 50-59, 1986. PubMed: 3014040 Buetow KH, et al. A detailed multipoint gene map of chromosome 1q. Genomics 8: 13-21, 1990. PubMed: 1981991 Klickstein LB, et al. Human C3b/C4b receptor (CR1). Demonstration of long homologous repeating domains that are composed of the short consensus repeats characteristic of C3/C4 binding proteins. J. Exp. Med. 165: 1095-1112, 1987. PubMed: 2951479 Barbara Weiffenbach, personal communication |
