| 产品名称： | pSY2501 |
|---|---|
| 商品货号： | TS154212 |
| Designations： | pSY2501 |
| GenBank Number： | I02222 |
| Species： | Homo sapiens, human |
| Depositors： | Cetus Corp., A Belt, Cetus Corp. |
| Applications： | produces protein interferon, beta 1, fibroblast |
| Vector： | Construct size (kb): 5.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pTRP3 Intact vector size: 4.460 Type of vector: plasmid Vector end: HindIII Vector end: BamHI Cloning sites: HindIII Polylinker sites: Other unique sites: BamHI Construction: pVH153, pBR322 Host range: Escherichia coli Features (with orientation and position when available): promoter: trp, -> ribosome-binding site: , -> restriction site: HindIII, -> marker(s): tetR, -> replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: human Gene symbol: IFNB1 Other gene symbols: IFNB Genomic copy number: unique Gene name: interferon, beta 1, fibroblast Contains complete coding sequence?: U Chromosome: 9; Localization: 9 p22 Type of DNA: cDNA Insert end: HindIII Insert end: XhoII Insert size (kb): 0.5 Cross references: DNA Seq. Acc.: I02222 Insert lengths(kb): 0.5 Gene product: interferon, beta 1, fibroblast IFNB Target Gene: interferon, beta 1, fibroblast |
| Insert Size (kb)： | 0.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Contains an Escherichia coli trp promoter regulating expression of complete coding sequence. Mutated codon changes Cys17 to Ser17. Contains 4 internal HinfI and 1 PstI sites. Constructed by ligating HindIII/XhoII fragment into HindIII/BamHI-cut vector (destroying XhoII/BamHI site); HindIII/XhoII digest gives nearly complete insert. |
| References： | Lin LS, et al. Human recombinant interleukin-2-muteins. US Patent 4,518,584 dated May 21 1985 Gelfand DH, et al. Polypeptide expression using a portable temperature sensitive control cassette with a positive retroregulatory element. US Patent 4,666,848 dated May 19 1987 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pSY2501 |
|---|---|
| 商品货号： | TS154212 |
| Designations： | pSY2501 |
| GenBank Number： | I02222 |
| Species： | Homo sapiens, human |
| Depositors： | Cetus Corp., A Belt, Cetus Corp. |
| Applications： | produces protein interferon, beta 1, fibroblast |
| Vector： | Construct size (kb): 5.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pTRP3 Intact vector size: 4.460 Type of vector: plasmid Vector end: HindIII Vector end: BamHI Cloning sites: HindIII Polylinker sites: Other unique sites: BamHI Construction: pVH153, pBR322 Host range: Escherichia coli Features (with orientation and position when available): promoter: trp, -> ribosome-binding site: , -> restriction site: HindIII, -> marker(s): tetR, -> replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: human Gene symbol: IFNB1 Other gene symbols: IFNB Genomic copy number: unique Gene name: interferon, beta 1, fibroblast Contains complete coding sequence?: U Chromosome: 9; Localization: 9 p22 Type of DNA: cDNA Insert end: HindIII Insert end: XhoII Insert size (kb): 0.5 Cross references: DNA Seq. Acc.: I02222 Insert lengths(kb): 0.5 Gene product: interferon, beta 1, fibroblast IFNB Target Gene: interferon, beta 1, fibroblast |
| Insert Size (kb)： | 0.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Contains an Escherichia coli trp promoter regulating expression of complete coding sequence. Mutated codon changes Cys17 to Ser17. Contains 4 internal HinfI and 1 PstI sites. Constructed by ligating HindIII/XhoII fragment into HindIII/BamHI-cut vector (destroying XhoII/BamHI site); HindIII/XhoII digest gives nearly complete insert. |
| References： | Lin LS, et al. Human recombinant interleukin-2-muteins. US Patent 4,518,584 dated May 21 1985 Gelfand DH, et al. Polypeptide expression using a portable temperature sensitive control cassette with a positive retroregulatory element. US Patent 4,666,848 dated May 19 1987 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
