VMM425

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货期:

编号:TS154286

品牌:Testobio

产品名称：	VMM425
商品货号：	TS154286
Organism：	Homo sapiens, human
Tissue：	Melanoma, Pelvic Soft Tissue Metastasis
Cell Type：	Melanocyte
Product Format：	frozen
Morphology：	Epithelial-like
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	Melanoma, Stage IV; malignant
Age：	60
Gender：	Male
Ethnicity：	Caucasian
Applications：	Drug screening Development of targeted therapy Development of combination therapy Tumor vaccine development
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	Derived from a pelvic soft tissue recurrence. Established from digest of Right Pelvic Tumor Metastasis in PETI Medium.
Clinical Data：	Primary Site: Left Foot (Plantar); Metastatic Site: Pelvic Soft Tissue
HLA Typing：	A2,A31,B7,B51,Cw7?,Cw16?,DR4(DRB10404),DR11(DRB11101) or DR13(DRB1131401),DR52(DRB30202),DR53(DRB4*0103)
Comments：	NRAS Mutation: Q61L Q61R CDKN2A: wt BRAF: wt PET Scan +
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing：	Volumes are for a T-75 flask; Adjust accordingly Remove and discard the cell culture medium from the flask. Rinse the cell monolayer with Dulbecco’s PBS without calcium or magnesium and remove. Add 3 to 4 ml of the trypsin-EDTA solution, rotate flask to rinse cell monolayer, remove trypsin solution, and incubate at 37^oC. Once the cells appear to be detached, add 10 ml of complete growth medium with a pipette to the cell suspension to inactivate the trypsin. Gently wash any remaining cells from the growth surface of the xa0 flask. Check the xa0cells with the microscope to be sure that most (>95%) are single cells. If cell clusters are apparent, continue to disperse the cells with gentle pipetting. Subculture as necessary. Place the flask back into the incubator. Examine the culture the following day to ensure the cells have reattached and are actively growing. Repeat when cells reach ~ 50-75% confluence. CELLS DO NOT GET CONFLUENT
Cryopreservation：	Fetal bovine serum, 90%; DMSO, 10%
Culture Conditions：	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂ ), 5%
STR Profile：	Amelogenin: X xa0 xa0xa0 D5S818: 11 D13S317: 9,13xa0 D7S820: 9 D16S539: 12 vWA: 17,19 TH01: 8,9.3 xa0 TPOX: 8,12 CSF1PO: 12
Sterility Tests：	Pass
Population Doubling Level (PDL)：	unknown
Population Doubling Capacity：	unknown
Name of Depositor：	Craig L. Slingluff, Jr. M.D.
Passage History：	Original deposit at one passage from the primary culture
Year of Origin：	2007
References：	Molhoek K, et al. Comprehensive analysis of RTK activation in human melanomas reveals autocrine signaling through IGF-1R. Melanoma Res 21(4): 274–284, 2011. PubMed: 21654344

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VMM425

货号: TS154286
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产品名称：	VMM425
商品货号：	TS154286
Organism：	Homo sapiens, human
Tissue：	Melanoma, Pelvic Soft Tissue Metastasis
Cell Type：	Melanocyte
Product Format：	frozen
Morphology：	Epithelial-like
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	Melanoma, Stage IV; malignant
Age：	60
Gender：	Male
Ethnicity：	Caucasian
Applications：	Drug screening Development of targeted therapy Development of combination therapy Tumor vaccine development
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	Derived from a pelvic soft tissue recurrence. Established from digest of Right Pelvic Tumor Metastasis in PETI Medium.
Clinical Data：	Primary Site: Left Foot (Plantar); Metastatic Site: Pelvic Soft Tissue
HLA Typing：	A2,A31,B7,B51,Cw7?,Cw16?,DR4(DRB10404),DR11(DRB11101) or DR13(DRB1131401),DR52(DRB30202),DR53(DRB4*0103)
Comments：	NRAS Mutation: Q61L Q61R CDKN2A: wt BRAF: wt PET Scan +
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing：	Volumes are for a T-75 flask; Adjust accordingly Remove and discard the cell culture medium from the flask. Rinse the cell monolayer with Dulbecco’s PBS without calcium or magnesium and remove. Add 3 to 4 ml of the trypsin-EDTA solution, rotate flask to rinse cell monolayer, remove trypsin solution, and incubate at 37^oC. Once the cells appear to be detached, add 10 ml of complete growth medium with a pipette to the cell suspension to inactivate the trypsin. Gently wash any remaining cells from the growth surface of the xa0 flask. Check the xa0cells with the microscope to be sure that most (>95%) are single cells. If cell clusters are apparent, continue to disperse the cells with gentle pipetting. Subculture as necessary. Place the flask back into the incubator. Examine the culture the following day to ensure the cells have reattached and are actively growing. Repeat when cells reach ~ 50-75% confluence. CELLS DO NOT GET CONFLUENT
Cryopreservation：	Fetal bovine serum, 90%; DMSO, 10%
Culture Conditions：	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂ ), 5%
STR Profile：	Amelogenin: X xa0 xa0xa0 D5S818: 11 D13S317: 9,13xa0 D7S820: 9 D16S539: 12 vWA: 17,19 TH01: 8,9.3 xa0 TPOX: 8,12 CSF1PO: 12
Sterility Tests：	Pass
Population Doubling Level (PDL)：	unknown
Population Doubling Capacity：	unknown
Name of Depositor：	Craig L. Slingluff, Jr. M.D.
Passage History：	Original deposit at one passage from the primary culture
Year of Origin：	2007
References：	Molhoek K, et al. Comprehensive analysis of RTK activation in human melanomas reveals autocrine signaling through IGF-1R. Melanoma Res 21(4): 274–284, 2011. PubMed: 21654344