| 产品名称： | H5.52 |
|---|---|
| 商品货号： | TS154537 |
| Designations： | H5.52 |
| Species： | Homo sapiens, human |
| Depositors： | MI Skraastad |
| Applications： | For detecting the RFLP, the complete insert should be used to get good signal. |
| Vector： | Construct size (kb): 10.0 |
| Insert： | DNA: genomic Insert lengths(kb): 3.599999904632568 Tissue: lymphoblast GM1416 (48,XXXX) cell line Gene product: DNA Segment, single copy D4S10 Alleles: K2, A1, A2, J2, K1, L1, L2, J1 |
| Insert Size (kb)： | 3.600 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/BglII--4.9, 2.45, 0.67, 0.48; BamHI--10.0; BglII--10.0; EcoRI--5.3, 2.65, 1.25; HindIII--10.0. The GM1416 cell line was incorrectly described as 49,XXXXY. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The distance between the EcoRI RFLP (detected by F.53, ATCC 61108) and the MspI RFLP (detected by H5.52, TS154537) is 48 kb. For detecting the RFLP, the complete insert should be used to get good signal. This probe was derived from the cosmid C5.5. C5.5 was isolated from a library constructed by digesting the vector C2RB with BamHI and SmaI and inserting size-selected fragments from GM1416 partially digested with MboI. This probe was derived from C5.5 by digesting with BglII and religating. This is from the more telomeric end of C5.5. The 3.6 kb DNA fragment in this clone contains an internal EcoRI site. An EcoRI/BglII digest of the clone gives 2 unique fragments from the insert: a 2.4 kb EcoRI fragment and a 1.2 kb EcoRI/BglII fragment. |
| References： | Skraastad MI, et al. Mapping of recombinants near the Huntington disease locus by using G8 (D4S10) and newly isolated markers in the D4S10 region. Am. J. Hum. Genet. 44: 560-566, 1989. PubMed: 2564732 Bakker E, et al. Two additional RFLPs at the D4S10 locus, useful for Huntingtons disease (HD)-family studies. Nucleic Acids Res. 15: 9100, 1987. PubMed: 2891108 Mette I Skraastad, personal communication |
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| 产品名称： | H5.52 |
|---|---|
| 商品货号： | TS154537 |
| Designations： | H5.52 |
| Species： | Homo sapiens, human |
| Depositors： | MI Skraastad |
| Applications： | For detecting the RFLP, the complete insert should be used to get good signal. |
| Vector： | Construct size (kb): 10.0 |
| Insert： | DNA: genomic Insert lengths(kb): 3.599999904632568 Tissue: lymphoblast GM1416 (48,XXXX) cell line Gene product: DNA Segment, single copy D4S10 Alleles: K2, A1, A2, J2, K1, L1, L2, J1 |
| Insert Size (kb)： | 3.600 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/BglII--4.9, 2.45, 0.67, 0.48; BamHI--10.0; BglII--10.0; EcoRI--5.3, 2.65, 1.25; HindIII--10.0. The GM1416 cell line was incorrectly described as 49,XXXXY. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The distance between the EcoRI RFLP (detected by F.53, ATCC 61108) and the MspI RFLP (detected by H5.52, TS154537) is 48 kb. For detecting the RFLP, the complete insert should be used to get good signal. This probe was derived from the cosmid C5.5. C5.5 was isolated from a library constructed by digesting the vector C2RB with BamHI and SmaI and inserting size-selected fragments from GM1416 partially digested with MboI. This probe was derived from C5.5 by digesting with BglII and religating. This is from the more telomeric end of C5.5. The 3.6 kb DNA fragment in this clone contains an internal EcoRI site. An EcoRI/BglII digest of the clone gives 2 unique fragments from the insert: a 2.4 kb EcoRI fragment and a 1.2 kb EcoRI/BglII fragment. |
| References： | Skraastad MI, et al. Mapping of recombinants near the Huntington disease locus by using G8 (D4S10) and newly isolated markers in the D4S10 region. Am. J. Hum. Genet. 44: 560-566, 1989. PubMed: 2564732 Bakker E, et al. Two additional RFLPs at the D4S10 locus, useful for Huntingtons disease (HD)-family studies. Nucleic Acids Res. 15: 9100, 1987. PubMed: 2891108 Mette I Skraastad, personal communication |
