94T778

规格:

货期:

编号:TS154648

品牌:Testobio

产品名称：	94T778
商品货号：	TS154648
Organism：	Homo sapiens, human
Tissue：	retroperitoneum; recurring
Product Format：	frozen
Morphology：	fibroblast-like
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	liposarcoma; well-differentiated
Age：	69 years
Gender：	female
Ethnicity：	Caucasian
Applications：	The MDM2 amplification present in this cell line can be used as a model for testing new drugs. This cell line is one of the rare liposarcoma cell lines available.
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	48~51,XX,-7,-22,+32~6r,+marcp10/49~52,XX,del(7)(q),-22, +3~6r,+marcp3 . ish (WCP1+;WCP12+). ish mar(WCP1+;WCP12+)
Images：
Derivation：	The 94T778 cell line is a well-differentiated liposarcoma derived from the retroperitoneum of a 69 year old female. It was established from the second recurrence of the tumor from the same individual from which the 93T449 cell line (ATCC CRL-3043) was derived.
Oncogene：	MDM2 (murine double minute 2); CDK4 (cyclin-dependent kinase 4); HMGA2 (high mobility group AT-hook 2)
Comments：	Karyotypic and molecular characterization of this liposarcoma demonstrates the presence of ring and large marker chromosomes containing MDM2, CDK4 and HMGA2 amplification.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. Discard supernatant. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended. Medium renewal: every 2 to 3 days
Cryopreservation：	Freeze medium: complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%
STR Profile：	CSF1PO: 10, 11 D13S317: 8, 14 D16S539: 12, 13 D5S818: 12 D7S820: 10 TH01: 8, 9 TPOX: 8, 11 vWA: 14, 17 Amelogenin: X
Name of Depositor：	F Pedeutour

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94T778

货号: TS154648
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产品名称：	94T778
商品货号：	TS154648
Organism：	Homo sapiens, human
Tissue：	retroperitoneum; recurring
Product Format：	frozen
Morphology：	fibroblast-like
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	liposarcoma; well-differentiated
Age：	69 years
Gender：	female
Ethnicity：	Caucasian
Applications：	The MDM2 amplification present in this cell line can be used as a model for testing new drugs. This cell line is one of the rare liposarcoma cell lines available.
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	48~51,XX,-7,-22,+32~6r,+marcp10/49~52,XX,del(7)(q),-22, +3~6r,+marcp3 . ish (WCP1+;WCP12+). ish mar(WCP1+;WCP12+)
Images：
Derivation：	The 94T778 cell line is a well-differentiated liposarcoma derived from the retroperitoneum of a 69 year old female. It was established from the second recurrence of the tumor from the same individual from which the 93T449 cell line (ATCC CRL-3043) was derived.
Oncogene：	MDM2 (murine double minute 2); CDK4 (cyclin-dependent kinase 4); HMGA2 (high mobility group AT-hook 2)
Comments：	Karyotypic and molecular characterization of this liposarcoma demonstrates the presence of ring and large marker chromosomes containing MDM2, CDK4 and HMGA2 amplification.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. Discard supernatant. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended. Medium renewal: every 2 to 3 days
Cryopreservation：	Freeze medium: complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%
STR Profile：	CSF1PO: 10, 11 D13S317: 8, 14 D16S539: 12, 13 D5S818: 12 D7S820: 10 TH01: 8, 9 TPOX: 8, 11 vWA: 14, 17 Amelogenin: X
Name of Depositor：	F Pedeutour