| 产品名称： | Human adenovirus 41 |
|---|---|
| 商品货号： | TS154759 |
| Classification： | Adenoviridae, Mastadenovirus, Human adenovirus F |
| Deposited As： | Adenovirus type 41 |
| Agent： | Human adenovirus 41 |
| Strain： | Tak (73-3544) |
| Applications： | Enteric Research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | Good immunogen in rabbits. No two-way cross serum neutralization or HA with 37 prototypes. Related to strain Dugan (79-18025) by serum neutralization and HAI. HA at low titer in rat red blood cells. No HA in rhesus monkey or human O type red blood cells. GenBank: DQ315364.2. |
| Effect on Host： | CPE, cell rounding, clumping, sloughing |
| Recommended Host： | 293 HEK-293 (ATCC® CRL-1573™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 2-7 days |
| Name of Depositor： | JC de Jong, National Institute for Public Health and the Environment, Netherlands |
| Special Collection： | ATCC |
| Source： | Feces from 14 month old with gastroenteritis, Netherlands, 1973 |
| References： | Brown M, et al. Diagnosis of fastidious enteric adenoviruses 40 and 41 in stool specimans. J. Clin. Microbiol. 20: 334-338, 1984. PubMed: 6490823 Hierholzer JC, et al. Evaluation of human adenoviruses 38, 39, 40, and 41 as new serotypes. Intervirology. 29: 1-10, 1988. PubMed: 3384629 Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904 de Jong JC, et al. Candidate adenoviruses 40 and 41: fastidious adenoviruses from human infant stool. J. Med. Virol. 11: 215-231, 1983. PubMed: 6306161 Ma Y, Mathews MB. Structure, function, and evolution of adenovirus-associated RNA: a phylogenetic approach. J. Virol. 70: 5083-5099, 1996. PubMed: 8764016 |
| Cross References： | Nucleotide (GenBank) : U52573 Human adenovirus type 41 virus-associated RNA pre-terminal protein (pTP) and 52,55K protein genes, partial cds. |
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| 产品名称： | Human adenovirus 41 |
|---|---|
| 商品货号： | TS154759 |
| Classification： | Adenoviridae, Mastadenovirus, Human adenovirus F |
| Deposited As： | Adenovirus type 41 |
| Agent： | Human adenovirus 41 |
| Strain： | Tak (73-3544) |
| Applications： | Enteric Research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | Good immunogen in rabbits. No two-way cross serum neutralization or HA with 37 prototypes. Related to strain Dugan (79-18025) by serum neutralization and HAI. HA at low titer in rat red blood cells. No HA in rhesus monkey or human O type red blood cells. GenBank: DQ315364.2. |
| Effect on Host： | CPE, cell rounding, clumping, sloughing |
| Recommended Host： | 293 HEK-293 (ATCC® CRL-1573™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 2-7 days |
| Name of Depositor： | JC de Jong, National Institute for Public Health and the Environment, Netherlands |
| Special Collection： | ATCC |
| Source： | Feces from 14 month old with gastroenteritis, Netherlands, 1973 |
| References： | Brown M, et al. Diagnosis of fastidious enteric adenoviruses 40 and 41 in stool specimans. J. Clin. Microbiol. 20: 334-338, 1984. PubMed: 6490823 Hierholzer JC, et al. Evaluation of human adenoviruses 38, 39, 40, and 41 as new serotypes. Intervirology. 29: 1-10, 1988. PubMed: 3384629 Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904 de Jong JC, et al. Candidate adenoviruses 40 and 41: fastidious adenoviruses from human infant stool. J. Med. Virol. 11: 215-231, 1983. PubMed: 6306161 Ma Y, Mathews MB. Structure, function, and evolution of adenovirus-associated RNA: a phylogenetic approach. J. Virol. 70: 5083-5099, 1996. PubMed: 8764016 |
| Cross References： | Nucleotide (GenBank) : U52573 Human adenovirus type 41 virus-associated RNA pre-terminal protein (pTP) and 52,55K protein genes, partial cds. |
