HFBEH24-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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HFBEH24
HFBEH24
规格:
货期:
编号:TS154767
品牌:Testobio
产品名称: HFBEH24
商品货号: TS154767
Designations: HFBEH24
GenBank Number:

T07326

Species: Homo sapiens, human
Vector:
Construct size (kb): 4.199999809265137
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: MDU1
Genomic copy number: unique
Gene name: antigen identified by monoclonal antibodies 4F2, TRA1.10,
TROP4, and T43
Contains complete coding sequence?: N
Chromosome: 11; Localization: 11 q12.1-q13.1
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.25
Cross references: DNA Seq. Acc.: J02769; EST: EST05215
Nucleotides 1-316 of the insert correspond to
nucleotides 1802-1486 of J02769 with 97% identity.
DNA Seq. Acc.: T07326; EST: EST05215; Primer: M13-21; THC
id: THC122463
Insert lengths(kb): 1.25
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Gene product: antigen identified by monoclonal antibodies 4F2, TRA1.10, TROP4, and T43 MDU1
Insert Size (kb): 1.25
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.25.
Based on the alignment with GenBank accession J02769 (1822 nt, CDS 54-1643) and the size of the insert, this clone appears to contain part of the coding sequence and the 3 untranslated region.
References:

Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434

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HFBEH24

  • 货号: TS154767
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: HFBEH24
商品货号: TS154767
Designations: HFBEH24
GenBank Number:

T07326

Species: Homo sapiens, human
Vector:
Construct size (kb): 4.199999809265137
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: MDU1
Genomic copy number: unique
Gene name: antigen identified by monoclonal antibodies 4F2, TRA1.10,
TROP4, and T43
Contains complete coding sequence?: N
Chromosome: 11; Localization: 11 q12.1-q13.1
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.25
Cross references: DNA Seq. Acc.: J02769; EST: EST05215
Nucleotides 1-316 of the insert correspond to
nucleotides 1802-1486 of J02769 with 97% identity.
DNA Seq. Acc.: T07326; EST: EST05215; Primer: M13-21; THC
id: THC122463
Insert lengths(kb): 1.25
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Gene product: antigen identified by monoclonal antibodies 4F2, TRA1.10, TROP4, and T43 MDU1
Insert Size (kb): 1.25
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.25.
Based on the alignment with GenBank accession J02769 (1822 nt, CDS 54-1643) and the size of the insert, this clone appears to contain part of the coding sequence and the 3 untranslated region.
References:

Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434

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