| 产品名称： | pHP45omega-Hg JF303 |
|---|---|
| 商品货号： | TS154973 |
| Designations： | pHP45omega-Hg JF303 |
| Depositors： | J Frey |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.0900001525878910 Vector: pHP45omega-Hg (plasmid) Construction: pHP45:omega, pME495 HgR Marker(s):HgR,ampR Construct size (kb): 7.090000152587891 Features: marker(s): ampR, HgR replicon: pMB1 terminator: T4D gene 32 |
| Applications： | integrating vector shuttle vector vector permitting positive selection for integration vector useful for interposon mutagenesis |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 2.6, 1.6; HindIII--5.4, 2.6; SalI--8.1. Because resistance levels may vary, it is important to determine the proper antibiotic concentration for each strain or isolate. One of a series (ATCC 37653 through 37657) of vectors for in vitro mutagenesis (via the omega interposon) of gram-negative bacteria. pHP45:omega was cut with BssHII + SphI (removing the spcR/strR gene) and the ends were made blunt. This was ligated to a blunt-ended 4 kb SmaI/BglII fragment of pME495 containing the mercury resistance operon of Tn501. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Fellay R, et al. Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of Gram-negative bacteria. Gene 52: 147-154, 1987. PubMed: 3038679 Frey J, Krisch HM. Omega mutagenesis in Gram-negative bacteria: a selectable interposon which is strongly polar in a wide range of bacterial species. Gene 36: 143-150, 1985. PubMed: 2998930 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | pHP45omega-Hg JF303 |
|---|---|
| 商品货号： | TS154973 |
| Designations： | pHP45omega-Hg JF303 |
| Depositors： | J Frey |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.0900001525878910 Vector: pHP45omega-Hg (plasmid) Construction: pHP45:omega, pME495 HgR Marker(s):HgR,ampR Construct size (kb): 7.090000152587891 Features: marker(s): ampR, HgR replicon: pMB1 terminator: T4D gene 32 |
| Applications： | integrating vector shuttle vector vector permitting positive selection for integration vector useful for interposon mutagenesis |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 2.6, 1.6; HindIII--5.4, 2.6; SalI--8.1. Because resistance levels may vary, it is important to determine the proper antibiotic concentration for each strain or isolate. One of a series (ATCC 37653 through 37657) of vectors for in vitro mutagenesis (via the omega interposon) of gram-negative bacteria. pHP45:omega was cut with BssHII + SphI (removing the spcR/strR gene) and the ends were made blunt. This was ligated to a blunt-ended 4 kb SmaI/BglII fragment of pME495 containing the mercury resistance operon of Tn501. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Fellay R, et al. Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of Gram-negative bacteria. Gene 52: 147-154, 1987. PubMed: 3038679 Frey J, Krisch HM. Omega mutagenesis in Gram-negative bacteria: a selectable interposon which is strongly polar in a wide range of bacterial species. Gene 36: 143-150, 1985. PubMed: 2998930 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |
