| 产品名称： | Quantitative Synthetic West Nile Virus RNA |
|---|---|
| 商品货号： | TS154978 |
| Agent： | Quantitative Synthetic West Nile Virus RNA |
| Applications： | ATCC® Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load. |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen powder Specification range: >1.0 x 108 copies |
| Storage Conditions： | 8°C or colder |
| Comments： | Genetic Target:xa0Preparation includes fragments from the 5UTR region, anchored capsid protein C gene, capsid protein C gene, membrane glycoprotein precursor prM gene, envelope protein E gene, nonstructural protein NS1, NS2A, NS3, and NS5 genes, and the 3 UTR region of the West Nile Virus genome. The length of the nucleic acid is approximately 1500 bp.xa0 Length: ~1500 bp Format Provided: Dried RNAxa0xa0 Starting Amount: 1.0 x 108 Copy Numbers (CN)xa0xa0 Downstream compatibility: Reverse Transcription PCR or other molecular applicationsxa0xa0 |
| Special Collection： | DNA |
| References： | Rondini S, et al. Development of multiplex PCR-ligase detection reaction assay for detection of West Nile virus. J Clin Microbiol 46(7): 2269-2279, 2008. PubMed: 18495862 Briese T, Glass W, Lipkin W. Detection of West Nile virus sequences in cerebrospinal fluid. Lancet 355(9215): 1614-1615, 2000 PubMed: 10821368 Eiden M, et al. Two new real-time quantitative reverse transcription polymerase chain reaction assays with unique target sites for the specific and sensitive detection of lineages 1 and 2 West Nile virus strains. J Vet Diagn Invest 22(5): 748-753, 2010 PubMed: 20807934 Jimenez-Clavero M, et al. A new fluorogenic real-time RT-PCR assay for detection of lineage 1 and lineage 2 West Nile viruses. J Vet Diagn Invest 18(5): 459-462, 2006 PubMed: 17037613 Lanciotti R, et al. Rapid detection of west nile virus from human clinical specimens, field-collected mosquitos, and avian samples by a TaqMan reverse transcriptase-PCR assay. J Clin Microbiol 38(11): 4066-4071, 2000 PubMed: 11060069 Papin J, Vahrson W, Dittmer D. SYBR green-based real-time quantitative PCR assay for detection of West Nile Virus circumvents false-negative results due to strain variability. J Clin Microbiol 42(4): 1511-1518, 2004 PubMed: 15070997 Parida M, et al. Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of West Nile virus. J Clin Microbiol 42(1): 257-263, 2004 PubMed: 14715762 Shi P, et al. High-throughput detection of West Nile virus RNA. J Clin Microbiol 39(4): 1264-1271, 2001 PubMed: 11283039 Usuku S, Noguchi Y, Takasaki T. Newly developed TaqMan assay to detect West Nile viruses in a wide range of viral strains. Jpn J Infect Dis 57(3): 129-130, 2004 PubMed: 15218227 |
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| 产品名称： | Quantitative Synthetic West Nile Virus RNA |
|---|---|
| 商品货号： | TS154978 |
| Agent： | Quantitative Synthetic West Nile Virus RNA |
| Applications： | ATCC® Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load. |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen powder Specification range: >1.0 x 108 copies |
| Storage Conditions： | 8°C or colder |
| Comments： | Genetic Target:xa0Preparation includes fragments from the 5UTR region, anchored capsid protein C gene, capsid protein C gene, membrane glycoprotein precursor prM gene, envelope protein E gene, nonstructural protein NS1, NS2A, NS3, and NS5 genes, and the 3 UTR region of the West Nile Virus genome. The length of the nucleic acid is approximately 1500 bp.xa0 Length: ~1500 bp Format Provided: Dried RNAxa0xa0 Starting Amount: 1.0 x 108 Copy Numbers (CN)xa0xa0 Downstream compatibility: Reverse Transcription PCR or other molecular applicationsxa0xa0 |
| Special Collection： | DNA |
| References： | Rondini S, et al. Development of multiplex PCR-ligase detection reaction assay for detection of West Nile virus. J Clin Microbiol 46(7): 2269-2279, 2008. PubMed: 18495862 Briese T, Glass W, Lipkin W. Detection of West Nile virus sequences in cerebrospinal fluid. Lancet 355(9215): 1614-1615, 2000 PubMed: 10821368 Eiden M, et al. Two new real-time quantitative reverse transcription polymerase chain reaction assays with unique target sites for the specific and sensitive detection of lineages 1 and 2 West Nile virus strains. J Vet Diagn Invest 22(5): 748-753, 2010 PubMed: 20807934 Jimenez-Clavero M, et al. A new fluorogenic real-time RT-PCR assay for detection of lineage 1 and lineage 2 West Nile viruses. J Vet Diagn Invest 18(5): 459-462, 2006 PubMed: 17037613 Lanciotti R, et al. Rapid detection of west nile virus from human clinical specimens, field-collected mosquitos, and avian samples by a TaqMan reverse transcriptase-PCR assay. J Clin Microbiol 38(11): 4066-4071, 2000 PubMed: 11060069 Papin J, Vahrson W, Dittmer D. SYBR green-based real-time quantitative PCR assay for detection of West Nile Virus circumvents false-negative results due to strain variability. J Clin Microbiol 42(4): 1511-1518, 2004 PubMed: 15070997 Parida M, et al. Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of West Nile virus. J Clin Microbiol 42(1): 257-263, 2004 PubMed: 14715762 Shi P, et al. High-throughput detection of West Nile virus RNA. J Clin Microbiol 39(4): 1264-1271, 2001 PubMed: 11283039 Usuku S, Noguchi Y, Takasaki T. Newly developed TaqMan assay to detect West Nile viruses in a wide range of viral strains. Jpn J Infect Dis 57(3): 129-130, 2004 PubMed: 15218227 |
