| 产品名称： | pBfm3 |
|---|---|
| 商品货号： | TS155389 |
| Designations： | pBfm3 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 6.199999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 3.5 Tissue: fetal muscle Gene product: utrophin (homologous to dystrophin)(utrophin) DMDL |
| Insert Size (kb)： | 3.5 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.5, 2.7; PstI--3.1, 3.1; HindIII/EcoRI--2.7, 1.9, 1.7; HindIII--4.3, 1.9. The insert contains the following restriction sites (approximate kb from the 5 end): HindIII--1.9; PstI--3.2; XbaI--3.2. The complete probe detects genomic EcoRI fragments of the following sizes (kb): human--15, 6.5, 3.7, 3.0, 1.8, 1.0, 0.3; mouse--16, 9.0, 4.0, 3.8, 1.0; Chinese hamster--7.5, 7.3, 2.0. The 5 1.9 kb EcoRI/HindIII fragment from the insert, that contains the 3 coding region of the transcript, detects human genomic PstI fragments of the following sites (kb): 19, 6.8, 5.0, 3.7, 3.0, and 1.8. The 3 1.6 kb HindIII/EcoRI fragment from the insert, that contains 3 untranslated sequences, detects genomic PstI fragments of the following sizes (kb): human--1.8; mouse--15; rat--7.0; hamster--7.0. Detects a transcript of 13 kb that is expressed ubiquitously. |
| References： | Love DR, et al. An autosomal transcript in skeletal muscle with homology to dystrophin. Nature 339: 55-58, 1989. PubMed: 2541343 Love DR, et al. Tissue distribution of the dystrophin-related gene product and expression in the mdx and dy mouse. Proc. Natl. Acad. Sci. USA 88: 3243-3247, 1991. PubMed: 2014247 |
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| 产品名称： | pBfm3 |
|---|---|
| 商品货号： | TS155389 |
| Designations： | pBfm3 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 6.199999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 3.5 Tissue: fetal muscle Gene product: utrophin (homologous to dystrophin)(utrophin) DMDL |
| Insert Size (kb)： | 3.5 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.5, 2.7; PstI--3.1, 3.1; HindIII/EcoRI--2.7, 1.9, 1.7; HindIII--4.3, 1.9. The insert contains the following restriction sites (approximate kb from the 5 end): HindIII--1.9; PstI--3.2; XbaI--3.2. The complete probe detects genomic EcoRI fragments of the following sizes (kb): human--15, 6.5, 3.7, 3.0, 1.8, 1.0, 0.3; mouse--16, 9.0, 4.0, 3.8, 1.0; Chinese hamster--7.5, 7.3, 2.0. The 5 1.9 kb EcoRI/HindIII fragment from the insert, that contains the 3 coding region of the transcript, detects human genomic PstI fragments of the following sites (kb): 19, 6.8, 5.0, 3.7, 3.0, and 1.8. The 3 1.6 kb HindIII/EcoRI fragment from the insert, that contains 3 untranslated sequences, detects genomic PstI fragments of the following sizes (kb): human--1.8; mouse--15; rat--7.0; hamster--7.0. Detects a transcript of 13 kb that is expressed ubiquitously. |
| References： | Love DR, et al. An autosomal transcript in skeletal muscle with homology to dystrophin. Nature 339: 55-58, 1989. PubMed: 2541343 Love DR, et al. Tissue distribution of the dystrophin-related gene product and expression in the mdx and dy mouse. Proc. Natl. Acad. Sci. USA 88: 3243-3247, 1991. PubMed: 2014247 |
