| 产品名称： | huB29/pUC19 |
|---|---|
| 商品货号： | TS155560 |
| Designations： | huB29/pUC19 |
| GenBank Number： | M80461 |
| Species： | Homo sapiens, human |
| Depositors： | B Mueller |
| Vector： | Construct size (kb): 3.910000085830689 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pUC19 Intact vector size: 2.686 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI HindIII Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI HindIII Construction: pUC71K Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ, MCS: EcoRI...HindIII, -> promoter: lac, replicon: pMB1, marker(s): ampR, Cross references: DNA Seq. Acc.: X02514 |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: IGB Genomic copy number: unique Gene name: immunoglobulin-associated beta (B29) Contains complete coding sequence?: Y Chromosome: 17; Localization: 17 q23 Tissue: tonsil Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 1.224 Cross references: DNA Seq. Acc.: M80461 Insert lengths(kb): 1.223999977111816 Tissue: tonsil Gene product: immunoglobulin-associated beta (B29) IGB |
| Insert Size (kb)： | 1.224 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--3.9; EcoRI--2.7, 1.2; BglI--1.45, 1.35; PstI--3.9; KpnI--3.7, 0.2. The insert contains the following restriction sites (nt from the 5 end): BglI--20, KpnI--117; NcoI--15, 1063; PvuII--81, 278, 464, 804. Contains the complete coding sequence (nt 15-702). |
| References： | Muller B, et al. Cloning and sequencing of the cDNA encoding the human homologue of the murine immunoglobulin-associated protein B29. Eur. J. Immunol. 22: 1621-1625, 1992. PubMed: 1534761 Brigitte Mueller, personal communication |
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| 产品名称： | huB29/pUC19 |
|---|---|
| 商品货号： | TS155560 |
| Designations： | huB29/pUC19 |
| GenBank Number： | M80461 |
| Species： | Homo sapiens, human |
| Depositors： | B Mueller |
| Vector： | Construct size (kb): 3.910000085830689 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pUC19 Intact vector size: 2.686 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI HindIII Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI HindIII Construction: pUC71K Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ, MCS: EcoRI...HindIII, -> promoter: lac, replicon: pMB1, marker(s): ampR, Cross references: DNA Seq. Acc.: X02514 |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: IGB Genomic copy number: unique Gene name: immunoglobulin-associated beta (B29) Contains complete coding sequence?: Y Chromosome: 17; Localization: 17 q23 Tissue: tonsil Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 1.224 Cross references: DNA Seq. Acc.: M80461 Insert lengths(kb): 1.223999977111816 Tissue: tonsil Gene product: immunoglobulin-associated beta (B29) IGB |
| Insert Size (kb)： | 1.224 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--3.9; EcoRI--2.7, 1.2; BglI--1.45, 1.35; PstI--3.9; KpnI--3.7, 0.2. The insert contains the following restriction sites (nt from the 5 end): BglI--20, KpnI--117; NcoI--15, 1063; PvuII--81, 278, 464, 804. Contains the complete coding sequence (nt 15-702). |
| References： | Muller B, et al. Cloning and sequencing of the cDNA encoding the human homologue of the murine immunoglobulin-associated protein B29. Eur. J. Immunol. 22: 1621-1625, 1992. PubMed: 1534761 Brigitte Mueller, personal communication |
