| 产品名称： | PRDII-BF1 |
|---|---|
| 商品货号： | TS155893 |
| Designations： | PRDII-BF1 |
| GenBank Number： | X51435 |
| Species： | Homo sapiens, human |
| Applications： | The encoded protein binds to the HIV-1 enhancer, the MHC H2-Kb promoter, and the immunoglobulin kappa enhancer. |
| Vector： | Construct size (kb): 13.5 |
| Insert： | DNA: cDNA Insert lengths(kb): 9.0 Gene product: human immunodeficiency virus type I enhancer-binding protein 1(zinc finger protein 40 (major histocompatibility complex binding protein 1, positive regulatory dom) ZNF40 Alleles: A1, A1, A1, A2, A2, A2 |
| Insert Size (kb)： | 9.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.1, 3.9, 2.8, 1.6; EcoRI--6.5, 2.7 (doublet), 1.6; HindIII--10.9, 1.8, 0.7; PstI--4.4, 2.2 (doublet), 1.3, 1.2 (doublet), 1.0 + smaller; XbaI--6.8, 4.3, 2.1. The insert contains the following restriction sites (nt position according to published sequence): BamHI--2067, 3633, 7529; EcoRI--2184, 3828, 6430; HindIII--1917, 2569, 2588. The encoded protein binds to the HIV-1 enhancer, the MHC H2-Kb promoter, and the immunoglobulin kappa enhancer. Detects genomic HindIII fragments of the following sizes (approximate kb): human--17, 4.9, 3.0; mouse--10. Extends from the NotI site at nt 152 (blunted) to a SmaI site (blunted) beyond nt 9015. Contains the complete coding sequence and a poly(A) tail of approximately 100 nt. The MspI polymorphism was detected using the 1.6 kb EcoRI fragment. |
| References： | Gaynor RB, et al. Localization of the zinc finger DNA-binding protein HIV-EP1/MBP-1/PRDII-BF1 to human chromosome 6p22.3-p24. Genomics 9: 758-761, 1991. PubMed: 2037300 Fan CM, Maniatis T. A DNA-binding protein containing two widely separated zinc finger motifs that recognize the same DNA sequence. Genes Dev. 4: 29-42, 1990. PubMed: 2106471 Fountain JW, et al. MspI RFLP detected by a ZNF-40 cDNA sequence. Nucleic Acids Res. 19: 2514, 1991. PubMed: 1710360 Chen-Ming Fan, personal communication |
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| 产品名称： | PRDII-BF1 |
|---|---|
| 商品货号： | TS155893 |
| Designations： | PRDII-BF1 |
| GenBank Number： | X51435 |
| Species： | Homo sapiens, human |
| Applications： | The encoded protein binds to the HIV-1 enhancer, the MHC H2-Kb promoter, and the immunoglobulin kappa enhancer. |
| Vector： | Construct size (kb): 13.5 |
| Insert： | DNA: cDNA Insert lengths(kb): 9.0 Gene product: human immunodeficiency virus type I enhancer-binding protein 1(zinc finger protein 40 (major histocompatibility complex binding protein 1, positive regulatory dom) ZNF40 Alleles: A1, A1, A1, A2, A2, A2 |
| Insert Size (kb)： | 9.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.1, 3.9, 2.8, 1.6; EcoRI--6.5, 2.7 (doublet), 1.6; HindIII--10.9, 1.8, 0.7; PstI--4.4, 2.2 (doublet), 1.3, 1.2 (doublet), 1.0 + smaller; XbaI--6.8, 4.3, 2.1. The insert contains the following restriction sites (nt position according to published sequence): BamHI--2067, 3633, 7529; EcoRI--2184, 3828, 6430; HindIII--1917, 2569, 2588. The encoded protein binds to the HIV-1 enhancer, the MHC H2-Kb promoter, and the immunoglobulin kappa enhancer. Detects genomic HindIII fragments of the following sizes (approximate kb): human--17, 4.9, 3.0; mouse--10. Extends from the NotI site at nt 152 (blunted) to a SmaI site (blunted) beyond nt 9015. Contains the complete coding sequence and a poly(A) tail of approximately 100 nt. The MspI polymorphism was detected using the 1.6 kb EcoRI fragment. |
| References： | Gaynor RB, et al. Localization of the zinc finger DNA-binding protein HIV-EP1/MBP-1/PRDII-BF1 to human chromosome 6p22.3-p24. Genomics 9: 758-761, 1991. PubMed: 2037300 Fan CM, Maniatis T. A DNA-binding protein containing two widely separated zinc finger motifs that recognize the same DNA sequence. Genes Dev. 4: 29-42, 1990. PubMed: 2106471 Fountain JW, et al. MspI RFLP detected by a ZNF-40 cDNA sequence. Nucleic Acids Res. 19: 2514, 1991. PubMed: 1710360 Chen-Ming Fan, personal communication |
