| 产品名称： | pHE-A1 he-A1 |
|---|---|
| 商品货号： | TS155995 |
| Designations： | pHE-A1 he-A1 |
| Species： | Homo sapiens, human |
| Depositors： | NK Spurr |
| Vector： | Construct size (kb): 8.100000381469727 |
| Insert： | DNA: genomic Insert lengths(kb): 3.900000095367432 Gene product: thyroid hormone receptor, alpha 1 (avian erythroblastic leukemia viral (v-erb-a) oncogene homolog 1, formerly ERBA1) ERBA1 Alleles: A1, A1, A2, A2 |
| Insert Size (kb)： | 3.900 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 4.2; HindIII--4.8, 2.8; BamHI--8.3. A 2.9 kb EcoRI/HindIII subfragment detects a PvuII RFLP. Insert includes 2.4 kb of unique sequence and a 1.5 kb HindIII/EcoRI fragment containing repetitive sequence. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. The PvuII polymorphism has demonstrated co-dominant segregation in 2 families. Only one allele is present in mouse/human hybrids with one chromosome 17. This is a subclone of lambdahe-A1 (p01239). Enzyme(s) not detecting polymorphism: BamHI, EcoRI, HindIII, HinfI, KpnI, PstI, TaqI. |
| References： | Jansson M, et al. Isolation and characterization of multiple human genes homologous to the oncogenes of avian erythroblastosis virus. EMBO J. 2: 561-565, 1983. PubMed: 6313346 Spurr NK, et al. Chromosomal localisation of the human homologues to the oncogenes erbA and B. EMBO J. 3: 159-163, 1984. PubMed: 6323162 Rider SH, et al. RFLP for the human erb-A1 gene. Nucleic Acids Res. 15: 863, 1987. PubMed: 2881264 Douglas JB, et al. A pulsed-field gel electrophoresis map locates the polymorphic probes for ERBA2 and ErbAbeta within 120 kb of each other, confirming that THRB (formerly ERBA2) maps to chromosome 3. Genomics 9: 398-400, 1991. PubMed: 1672299 Barbara Weiffenbach, personal communication |
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| 产品名称： | pHE-A1 he-A1 |
|---|---|
| 商品货号： | TS155995 |
| Designations： | pHE-A1 he-A1 |
| Species： | Homo sapiens, human |
| Depositors： | NK Spurr |
| Vector： | Construct size (kb): 8.100000381469727 |
| Insert： | DNA: genomic Insert lengths(kb): 3.900000095367432 Gene product: thyroid hormone receptor, alpha 1 (avian erythroblastic leukemia viral (v-erb-a) oncogene homolog 1, formerly ERBA1) ERBA1 Alleles: A1, A1, A2, A2 |
| Insert Size (kb)： | 3.900 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 4.2; HindIII--4.8, 2.8; BamHI--8.3. A 2.9 kb EcoRI/HindIII subfragment detects a PvuII RFLP. Insert includes 2.4 kb of unique sequence and a 1.5 kb HindIII/EcoRI fragment containing repetitive sequence. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. The PvuII polymorphism has demonstrated co-dominant segregation in 2 families. Only one allele is present in mouse/human hybrids with one chromosome 17. This is a subclone of lambdahe-A1 (p01239). Enzyme(s) not detecting polymorphism: BamHI, EcoRI, HindIII, HinfI, KpnI, PstI, TaqI. |
| References： | Jansson M, et al. Isolation and characterization of multiple human genes homologous to the oncogenes of avian erythroblastosis virus. EMBO J. 2: 561-565, 1983. PubMed: 6313346 Spurr NK, et al. Chromosomal localisation of the human homologues to the oncogenes erbA and B. EMBO J. 3: 159-163, 1984. PubMed: 6323162 Rider SH, et al. RFLP for the human erb-A1 gene. Nucleic Acids Res. 15: 863, 1987. PubMed: 2881264 Douglas JB, et al. A pulsed-field gel electrophoresis map locates the polymorphic probes for ERBA2 and ErbAbeta within 120 kb of each other, confirming that THRB (formerly ERBA2) maps to chromosome 3. Genomics 9: 398-400, 1991. PubMed: 1672299 Barbara Weiffenbach, personal communication |
