Chilodonella uncinata-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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Chilodonella uncinata
Chilodonella uncinata
规格:
货期:
编号:TS156013
品牌:Testobio
产品名称： Chilodonella uncinata Ehrenberg
商品货号： TS156013
Deposited As： Chilodonella uncinata Ehrenberg
Strain Designations： ATCC:0189:1
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation： Contaminant of Euplotes gracilis culture, ATCC 50191, 1988
Product Format： frozen
Storage Conditions： Frozen: -70°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Protocols Section
Type Strain： no
Medium： ATCC® Medium 802: Sonneborns Paramecium medium
Growth Conditions：
Temperature: 25°C
Culture System:xa0Grown with Enterobacter aerogenes ATCC 13048 and mixed bacteria
Cryopreservation： Reagents
Cryoprotective Solution
DMSO, 1.5 mL
Fresh growth medium w/o bacteria, 8.5 mL

Harvest and Preservation:
  1. Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat.xa0 Allow to cool.
  2. Harvest cells from a culture in stationary phase (1-2 days after reaching peak density).
  3. Gently discard most of the supernatant and vigorously agitate the flasks to detach the cells.xa0
  4. Determine the cell concentration using a hemacytometer.xa0xa0 Adjust the concentration to 2 x 105/mL in fresh medium.xa0 If the concentration is too low, centrifuge at 200 x g for 5 minutes and resuspend the pellet with the supernatant to the desired volume.xa0
  5. Mix the cell preparation and the cryoprotective solution in equal portions.
  6. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  7. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.) xa0
  8. Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.
  9. To establish a culture from the frozen place the vial in a 35°C water bath.xa0 Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.
  10. Immediately after thawing, do not leave in water bath, aseptically remove the contents of the ampule and inoculate the entire contents into a T-25 flask containing 10 mL of bacterized ATCC medium 802.
  11. Incubate at 25°C with the cap on loosely.
  12. Once the culture is established, follow the protocol for maintenance of culture.
Name of Depositor： TA Nerad
Year of Origin： 1988
References：

Grant JR, et al. Gene discovery from a pilot study of the transcriptomes from three diverse microbial eukaryotes: Corallomyxa tenera, Chilodonella uncinata, and Subulatomonas tetraspora. Protist Genomics 1: 3-18, 2012.

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Chilodonella uncinata

  • 货号: TS156013
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称： Chilodonella uncinata Ehrenberg
商品货号： TS156013
Deposited As： Chilodonella uncinata Ehrenberg
Strain Designations： ATCC:0189:1
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation： Contaminant of Euplotes gracilis culture, ATCC 50191, 1988
Product Format： frozen
Storage Conditions： Frozen: -70°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Protocols Section
Type Strain： no
Medium： ATCC® Medium 802: Sonneborns Paramecium medium
Growth Conditions：
Temperature: 25°C
Culture System:xa0Grown with Enterobacter aerogenes ATCC 13048 and mixed bacteria
Cryopreservation： Reagents
Cryoprotective Solution
DMSO, 1.5 mL
Fresh growth medium w/o bacteria, 8.5 mL

Harvest and Preservation:
  1. Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat.xa0 Allow to cool.
  2. Harvest cells from a culture in stationary phase (1-2 days after reaching peak density).
  3. Gently discard most of the supernatant and vigorously agitate the flasks to detach the cells.xa0
  4. Determine the cell concentration using a hemacytometer.xa0xa0 Adjust the concentration to 2 x 105/mL in fresh medium.xa0 If the concentration is too low, centrifuge at 200 x g for 5 minutes and resuspend the pellet with the supernatant to the desired volume.xa0
  5. Mix the cell preparation and the cryoprotective solution in equal portions.
  6. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  7. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.) xa0
  8. Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.
  9. To establish a culture from the frozen place the vial in a 35°C water bath.xa0 Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.
  10. Immediately after thawing, do not leave in water bath, aseptically remove the contents of the ampule and inoculate the entire contents into a T-25 flask containing 10 mL of bacterized ATCC medium 802.
  11. Incubate at 25°C with the cap on loosely.
  12. Once the culture is established, follow the protocol for maintenance of culture.
Name of Depositor： TA Nerad
Year of Origin： 1988
References：

Grant JR, et al. Gene discovery from a pilot study of the transcriptomes from three diverse microbial eukaryotes: Corallomyxa tenera, Chilodonella uncinata, and Subulatomonas tetraspora. Protist Genomics 1: 3-18, 2012.

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