| 产品名称： | pUK21-NotI |
|---|---|
| 商品货号： | TS156095 |
| Designations： | pUK21-NotI |
| Depositors： | DJ Stillman |
| Biosafety Level： | 1 |
| Host： | Escherichia coli HB101 |
| Vector Information： | Intact vector size: 3.250
Type of vector: plasmid
Cloning sites: StuI XhoI HindIII BsiWI PstI SalI PmeI SacI EcoRI SfiI XbaI Polylinker sites: SpeI StuI XhoI EcoRV NotI PvuII HindIII BsiWI PstI SalI PmeIxa0SacI EcoRV SpeI SfiI NotI XbaI SpeI ClaI Construction: pUC21 and pFA6b Features (with orientation and position when available): Vector: pUK21-NotI (plasmid)
Construction: pUC21 and pFA6b
Marker(s): kanR
Construct size (kb): 3.25 |
| Applications： | Cloning vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRV--3.25; XbaI--3.25; PvuI--2.0, 1.25. This plasmid is derived from pUC21 by deletion of the BamHI-BglII fragment and insertion of the NotI polylinker fragment from pFA6b. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37°C |
| References： | Vieira J. New pUC-derived cloning vectors with different selectable markers and DNA replication origins. Gene 100: 189-194, 1991. PubMed: 1905257 Wach A, et al. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae.. Yeast 10(13): 1793-1808, 1994. PubMed: 7747518 David J Stillman, personal communication |
| Shipped： | freeze-dried |
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| 产品名称： | pUK21-NotI |
|---|---|
| 商品货号： | TS156095 |
| Designations： | pUK21-NotI |
| Depositors： | DJ Stillman |
| Biosafety Level： | 1 |
| Host： | Escherichia coli HB101 |
| Vector Information： | Intact vector size: 3.250
Type of vector: plasmid
Cloning sites: StuI XhoI HindIII BsiWI PstI SalI PmeI SacI EcoRI SfiI XbaI Polylinker sites: SpeI StuI XhoI EcoRV NotI PvuII HindIII BsiWI PstI SalI PmeIxa0SacI EcoRV SpeI SfiI NotI XbaI SpeI ClaI Construction: pUC21 and pFA6b Features (with orientation and position when available): Vector: pUK21-NotI (plasmid)
Construction: pUC21 and pFA6b
Marker(s): kanR
Construct size (kb): 3.25 |
| Applications： | Cloning vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRV--3.25; XbaI--3.25; PvuI--2.0, 1.25. This plasmid is derived from pUC21 by deletion of the BamHI-BglII fragment and insertion of the NotI polylinker fragment from pFA6b. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37°C |
| References： | Vieira J. New pUC-derived cloning vectors with different selectable markers and DNA replication origins. Gene 100: 189-194, 1991. PubMed: 1905257 Wach A, et al. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae.. Yeast 10(13): 1793-1808, 1994. PubMed: 7747518 David J Stillman, personal communication |
| Shipped： | freeze-dried |
