L-alpha-2A L-cells-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

你好，请登录   免费注册    |    收藏本站
联系电话: 0574-87917803
你好,请登录   免费注册 | 收藏
联系电话: 0574-87917803
热门搜索: 金黄色葡萄球菌 铜绿假单胞菌 红菇 香菇
L-alpha-2A L-cells
L-alpha-2A L-cells
规格:
货期:
编号:TS156324
品牌:Testobio
产品名称： L-alpha-2A L-cells
商品货号： TS156324
Organism： Mus musculus, mouse
Product Format： frozen
Morphology： fibroblast
Culture Properties： adherent, single cells and loosely attached clusters
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age： 100 days
Gender： male
Strain： C3H/An
Storage Conditions： liquid nitrogen vapor phase
Disclosure： This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Images：
Derivation：
This line is a derivative of L-M(TK-) (see ATCC CCL-1.3) that is stably transfected with the gene for the human adrenergic alpha2A receptor.
Clinical Data：
male
Receptor Expression：
human adrenergic alpha2A, expressed
Comments：
This line is a derivative of L-M(TK-) (see ATCC CCL-1.3) that is stably transfected with the gene for the human adrenergic alpha2A receptor.
Complete Growth Medium： The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Two to three times weekly
Cryopreservation：
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions：
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Name of Depositor： Synaptic Pharmaceutical Corporation
Deposited As： Mus musculus
U.S. Patent Number：
References：

Gluchowski C, et al. Use of alpha1C specific compounds to treat benign prostatic hyperplasia. US Patent 5,403,847 dated Apr 4 1995

Lagu B, et al. Morpholinone and morpholine derivatives and uses thereof. US Patent 6,531,471 dated Mar 11 2003

Gluchowski C, et al. Use of .alpha.1C specific compounds to treat benign prostatic hyperplasia. US Patent 6,602,888 dated Aug 5 2003

Cui D, et al. Dihydropyrimidines and uses thereof . U.S. Patent 6,680,323 dated Jan 20 2004

首页 > 产品中心 > 微生物培养 > 菌株 > null > L-alpha-2A L-cells

产品中心 Product Center

联系我们CONTACT US

  • 0574-87917803
    24小时服务热线
  • testobio@163.com
    销售邮箱
  • 浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
    地址

联系方式

  • 章丽敏
    17757489010
  • 朱静
    18067106081
  • 李晴
    18067408036
  • 李晶晶
    13375187189
  • 周雪
    13372109301

L-alpha-2A L-cells

  • 货号: TS156324
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称： L-alpha-2A L-cells
商品货号： TS156324
Organism： Mus musculus, mouse
Product Format： frozen
Morphology： fibroblast
Culture Properties： adherent, single cells and loosely attached clusters
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age： 100 days
Gender： male
Strain： C3H/An
Storage Conditions： liquid nitrogen vapor phase
Disclosure： This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Images：
Derivation：
This line is a derivative of L-M(TK-) (see ATCC CCL-1.3) that is stably transfected with the gene for the human adrenergic alpha2A receptor.
Clinical Data：
male
Receptor Expression：
human adrenergic alpha2A, expressed
Comments：
This line is a derivative of L-M(TK-) (see ATCC CCL-1.3) that is stably transfected with the gene for the human adrenergic alpha2A receptor.
Complete Growth Medium： The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Two to three times weekly
Cryopreservation：
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions：
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Name of Depositor： Synaptic Pharmaceutical Corporation
Deposited As： Mus musculus
U.S. Patent Number：
References：

Gluchowski C, et al. Use of alpha1C specific compounds to treat benign prostatic hyperplasia. US Patent 5,403,847 dated Apr 4 1995

Lagu B, et al. Morpholinone and morpholine derivatives and uses thereof. US Patent 6,531,471 dated Mar 11 2003

Gluchowski C, et al. Use of .alpha.1C specific compounds to treat benign prostatic hyperplasia. US Patent 6,602,888 dated Aug 5 2003

Cui D, et al. Dihydropyrimidines and uses thereof . U.S. Patent 6,680,323 dated Jan 20 2004

扫码关注小红书账号
关注我们
联系电话：0574-87917803
章丽敏：17757489010
朱静：18067106081
李晴：18067408036
李晶晶：13375187189
周雪：13372109301
邮箱：testobio@163.com
地址：浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
在线留言
合作单位: