pLEX1B [WM2184]-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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pLEX1B [WM2184]
pLEX1B [WM2184]
规格:
货期:
编号:TS156332
品牌:Testobio
产品名称: pLEX1B WM2184
商品货号: TS156332
Designations: pLEX1B WM2184
Depositors: W Messer
Biosafety Level: 1
Vector Information:
Size (kb): 5.2909998893737790
Vector: pLEX1B (plasmid)
Construction: pT7-7, pNM480
Marker(s):ampR
Construct size (kb): 5.290999889373779
Features: marker(s): ampR
replicon: pMB1
reporter group: lacZ
MCS: XmnI...HindIII
ribosome-binding site: Shine-Dalgarno
Applications:
expression vector
vector permitting construction of fusion proteins beta-galactosidase beta-D-galactosidase
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--5.3; HindIII--5.3; PstI--5.3; XmnI--5.3.
Expression vector with efficient prokaryotic expression signals upstream of the XmnI site. Permits generation of a translational fusion with lacZ. pLEX1B, pLEX2B, and pLEX3B (TS156332-87200) differ in the reading frame established at the XmnI site.
Expression requires fusion of a 5TG of the insert with the A nucleotide of the XmnI site of the vector.
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions:
Temperature: 37.0°C
References:

Diederich L, et al. A versatile plasmid vector system for the regulated expression of genes in Escherichia coli. BioTechniques 16: 916-920, 1994. PubMed: 8068348

Shipped: freeze-dried
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pLEX1B [WM2184]

  • 货号: TS156332
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: pLEX1B WM2184
商品货号: TS156332
Designations: pLEX1B WM2184
Depositors: W Messer
Biosafety Level: 1
Vector Information:
Size (kb): 5.2909998893737790
Vector: pLEX1B (plasmid)
Construction: pT7-7, pNM480
Marker(s):ampR
Construct size (kb): 5.290999889373779
Features: marker(s): ampR
replicon: pMB1
reporter group: lacZ
MCS: XmnI...HindIII
ribosome-binding site: Shine-Dalgarno
Applications:
expression vector
vector permitting construction of fusion proteins beta-galactosidase beta-D-galactosidase
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--5.3; HindIII--5.3; PstI--5.3; XmnI--5.3.
Expression vector with efficient prokaryotic expression signals upstream of the XmnI site. Permits generation of a translational fusion with lacZ. pLEX1B, pLEX2B, and pLEX3B (TS156332-87200) differ in the reading frame established at the XmnI site.
Expression requires fusion of a 5TG of the insert with the A nucleotide of the XmnI site of the vector.
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions:
Temperature: 37.0°C
References:

Diederich L, et al. A versatile plasmid vector system for the regulated expression of genes in Escherichia coli. BioTechniques 16: 916-920, 1994. PubMed: 8068348

Shipped: freeze-dried
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