MOVAS

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货期:

编号:TS156341

品牌:Testobio

产品名称：	MOVAS
商品货号：	TS156341
Organism：	Mus musculus, mouse
Tissue：	aorta/smooth muscle
Cell Type：	immortalized with SV40 large T antigen
Product Format：	frozen
Morphology：	spindle-shaped
Culture Properties：	adherent
Biosafety Level：	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age：	adult
Strain：	C57BL/6
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	Primary vascular aortic smooth muscle cells isolated by collagenase - elastase digestions in December 1999 were transduced with retrovirus encoding the SV40 large T antigen as well as the gene for neomycin resistance. Clones were selected in the presence of 0.4 mg/ml G418 and subcloned by limiting dilution.
Genes Expressed：	actin; smooth muscle alpha actin,calponin 1; basic; smooth muscle,desmin,smooth muscle protein 22 (SM22 alpha)
Cellular Products：	actin; smooth muscle alpha actin calponin 1; basic; smooth muscle desmin smooth muscle protein 22 (SM22 alpha)
Comments：	Primary vascular aortic smooth muscle cells isolated by collagenase - elastase digestions in December 1999 were transduced with retrovirus encoding the SV40 large T antigen as well as the gene for neomycin resistance. Clones were selected in the presence of 0.4 mg/ml G418 and subcloned by limiting dilution.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 0.2 mg/ml G -418 fetal bovine serum to a final concentration of 10%
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping, do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by pipetting gently. Add appropriate aliquots of cell suspension to new culture vessels. An inoculum ofxa04 x 10³ toxa02 x 10⁴ viable cells/cm² is recommended. Maintain cultures at a cell concentration between 1 X 10⁴ and 1 X 10⁵ cells/cm² Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal: Two to three times weekly Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells: a Manual of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
Population Doubling Time：	15 hours
Name of Depositor：	M Husain
Year of Origin：	December, 1999
References：	Afroze T, Husain M. c-Myb-binding sites mediate G(1)/S-associated repression of the plasma membrane Ca(2+)-ATPase-1 promoter. J. Biol. Chem. 275: 9062-9069, 2000. PubMed: 10722757 Afroze T, et al. Calcineurin-independent regulation of plasma membrane Ca2+ ATPase-4 in the vascular smooth muscle cell cycle. Am. J. Physiol. Cell Physiol. : C88-C95, 2003. PubMed: 12660151

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MOVAS

货号: TS156341
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产品名称：	MOVAS
商品货号：	TS156341
Organism：	Mus musculus, mouse
Tissue：	aorta/smooth muscle
Cell Type：	immortalized with SV40 large T antigen
Product Format：	frozen
Morphology：	spindle-shaped
Culture Properties：	adherent
Biosafety Level：	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age：	adult
Strain：	C57BL/6
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	Primary vascular aortic smooth muscle cells isolated by collagenase - elastase digestions in December 1999 were transduced with retrovirus encoding the SV40 large T antigen as well as the gene for neomycin resistance. Clones were selected in the presence of 0.4 mg/ml G418 and subcloned by limiting dilution.
Genes Expressed：	actin; smooth muscle alpha actin,calponin 1; basic; smooth muscle,desmin,smooth muscle protein 22 (SM22 alpha)
Cellular Products：	actin; smooth muscle alpha actin calponin 1; basic; smooth muscle desmin smooth muscle protein 22 (SM22 alpha)
Comments：	Primary vascular aortic smooth muscle cells isolated by collagenase - elastase digestions in December 1999 were transduced with retrovirus encoding the SV40 large T antigen as well as the gene for neomycin resistance. Clones were selected in the presence of 0.4 mg/ml G418 and subcloned by limiting dilution.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 0.2 mg/ml G -418 fetal bovine serum to a final concentration of 10%
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping, do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by pipetting gently. Add appropriate aliquots of cell suspension to new culture vessels. An inoculum ofxa04 x 10³ toxa02 x 10⁴ viable cells/cm² is recommended. Maintain cultures at a cell concentration between 1 X 10⁴ and 1 X 10⁵ cells/cm² Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal: Two to three times weekly Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells: a Manual of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
Population Doubling Time：	15 hours
Name of Depositor：	M Husain
Year of Origin：	December, 1999
References：	Afroze T, Husain M. c-Myb-binding sites mediate G(1)/S-associated repression of the plasma membrane Ca(2+)-ATPase-1 promoter. J. Biol. Chem. 275: 9062-9069, 2000. PubMed: 10722757 Afroze T, et al. Calcineurin-independent regulation of plasma membrane Ca2+ ATPase-4 in the vascular smooth muscle cell cycle. Am. J. Physiol. Cell Physiol. : C88-C95, 2003. PubMed: 12660151