| 产品名称： | IB800 |
|---|---|
| 商品货号： | TS157012 |
| Designations： | IB800 |
| GenBank Number： | T03725 |
| Species： | Homo sapiens, human |
| Applications： | The primers can also be used to generate sequence from the 3 or 5 ends, respectively. |
| Vector： | Construct size (kb): 4.800000190734863 DESCRIPTION OF VECTOR COMPONENT: Name of vector: lafmid BA Intact vector size: 3.153 Type of vector: phagemid Vector end: HindIII Vector end: NotI Cloning sites: HindIII, BamHI, NotI, EcoRI Polylinker sites: HindIII, BamHI, NotI, EcoRI Other unique sites: ClaI, XmnI, ScaI, ApaLI Construction: pGEM Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, insert detection: lacZ, primer site: M13 universal, -> MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098 primer site: M13 reverse, promoter: lac, replicon: pMB1, marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: D0S8933E Gene name: DNA Segment, expressed Contains complete coding sequence?: U Tissue: infant brain, 3 months old (from Bento Soares) Type of DNA: cDNA Insert 5 end: Modification: HindIII Insert 3 end: Modification: NotI Insert size (kb): 1.5 Cross references: DNA Seq. Acc.: T03725 Insert lengths(kb): 1.5 Tissue: infant brain, 3 months old (from Bento Soares) Gene product: DNA Segment, expressed D0S8933E |
| Insert Size (kb)： | 1.5 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--uncut; ClaI--4.6; HindIII/NotI-- 3.1, 0.9, 0.6; PvuII--2.8, 1.3, 0.31; ScaI--4.6; EcoRI--4.6. Derived from a directionally cloned library with an average insert size of 1.7 kb. Inserts can be amplified with the M13 universal (forward) and reverse primers. The primers can also be used to generate sequence from the 3 or 5 ends, respectively. |
| References： | Khan AS, et al. Single pass sequencing and physical and genetic mapping of human brain cDNAs. Nat. Genet. 2: 180-185, 1992. PubMed: 1345165 Soares MB, et al. Construction and characterization of a normalized cDNA library. Proc. Natl. Acad. Sci. USA 91: 9228-9232, 1994. PubMed: 7937745 Marcelo B Soares, personal communication |
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13372109301
| 产品名称： | IB800 |
|---|---|
| 商品货号： | TS157012 |
| Designations： | IB800 |
| GenBank Number： | T03725 |
| Species： | Homo sapiens, human |
| Applications： | The primers can also be used to generate sequence from the 3 or 5 ends, respectively. |
| Vector： | Construct size (kb): 4.800000190734863 DESCRIPTION OF VECTOR COMPONENT: Name of vector: lafmid BA Intact vector size: 3.153 Type of vector: phagemid Vector end: HindIII Vector end: NotI Cloning sites: HindIII, BamHI, NotI, EcoRI Polylinker sites: HindIII, BamHI, NotI, EcoRI Other unique sites: ClaI, XmnI, ScaI, ApaLI Construction: pGEM Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, insert detection: lacZ, primer site: M13 universal, -> MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098 primer site: M13 reverse, promoter: lac, replicon: pMB1, marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: D0S8933E Gene name: DNA Segment, expressed Contains complete coding sequence?: U Tissue: infant brain, 3 months old (from Bento Soares) Type of DNA: cDNA Insert 5 end: Modification: HindIII Insert 3 end: Modification: NotI Insert size (kb): 1.5 Cross references: DNA Seq. Acc.: T03725 Insert lengths(kb): 1.5 Tissue: infant brain, 3 months old (from Bento Soares) Gene product: DNA Segment, expressed D0S8933E |
| Insert Size (kb)： | 1.5 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--uncut; ClaI--4.6; HindIII/NotI-- 3.1, 0.9, 0.6; PvuII--2.8, 1.3, 0.31; ScaI--4.6; EcoRI--4.6. Derived from a directionally cloned library with an average insert size of 1.7 kb. Inserts can be amplified with the M13 universal (forward) and reverse primers. The primers can also be used to generate sequence from the 3 or 5 ends, respectively. |
| References： | Khan AS, et al. Single pass sequencing and physical and genetic mapping of human brain cDNAs. Nat. Genet. 2: 180-185, 1992. PubMed: 1345165 Soares MB, et al. Construction and characterization of a normalized cDNA library. Proc. Natl. Acad. Sci. USA 91: 9228-9232, 1994. PubMed: 7937745 Marcelo B Soares, personal communication |
